Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA031857

Local Sample ID:	HF13
Subject ID:	SU000611
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000611
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
HF13	SA031857	FL007199	Heart failure	Group

Collection:

Collection ID:	CO000605
Collection Summary:	The samples were collected in Dr. Bruce Johnson lab, at the Department of Cardiovascular Diseases, Mayo Clinic. EBC samples were collected using Jaeger EcoScreen cooling unit (-20°C). Patients were breathing through mouth piece while wearing a nose clip during 30 minutes. Saliva was collected from the same patients during last 15 minutes. The samples were frozen immediately after collection.
Sample Type:	Saliva

Treatment:

Treatment ID:	TR000625
Treatment Summary:	We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points.

Treatment ID:

TR000625

Treatment Summary:

We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points.

Sample Preparation:

Sampleprep ID:	SP000618
Sampleprep Summary:	Saliva: Allow samples to thaw on ice at 4 °C for 30–60 min. Transfer 250 μl aliquots of saliva samples into 1.5ml EP tubes, centrifuge each sample at 13,300g for 10 min. Transfer 200μl of supernatant of saliva samples into 1.5 ml EP tubes, add 150 μl of phosphate buffer and 150 μl of 4mM TSP-d4 solution in D2O. Vortex for 20 s. Transfer the solution to 5 mm NMR tube.

Analysis:

MB Sample ID:	SA031857
Analysis ID:	AN000903
Analysis Type:	NMR
Num Factors:	2
Num Metabolites:	48
Units:	uM

NMR:

NMR ID:	NM000101
Analysis ID:	AN000903
Instrument Name:	Bruker 600 MHz Avance III HD spectrometer
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	600 MHz