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MB Sample ID: SA031910
| Local Sample ID: | 13-100C |
| Subject ID: | SU000612 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 60 |
| Gender: | male |
| Human Race: | Caucasian |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000612 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 60 |
| Gender: | male |
| Human Race: | Caucasian |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 13-100C | SA031910 | FL007201 | 100 uL | Plasma Volume Extracted |
Collection:
| Collection ID: | CO000606 |
| Collection Summary: | Plasma from blood drawn intravenously |
| Sample Type: | Blood |
| Collection Method: | IV |
| Additives: | EDTA |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000626 |
| Treatment Summary: | no treatment |
Sample Preparation:
| Sampleprep ID: | SP000619 |
| Sampleprep Summary: | Protein precipitation and drying followed by derivatization via methoxyamine and MSTFA |
| Extraction Method: | Protein precipitation |
| Sample Derivatization: | 18 mg/mL methoxyamine hydrochloride in pyridine for 30 min at 50°C, followed by trimethylsilylation with N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA) for 30 min at 50°C |
| Sample Spiking: | retention-time-lock internal standard of perdeuterated myristic acid |
Combined analysis:
| Analysis ID | AN000904 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB5-MS (15m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975B |
| Ion Mode | POSITIVE |
| Units | Peak area (Log2 transformed) |
Chromatography:
| Chromatography ID: | CH000642 |
| Chromatography Summary: | GC/MS methods follow previous studies using a 6890 N GC connected to a 5975B Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122–5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB5-MS (15m x 0.25mm,0.25um) |
| Flow Rate: | 2.0 mL/min |
| Time Program: | initial GC oven temperature of 60°C increased at 10°C/min to a final temperature of 325°C |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000804 |
| Analysis ID: | AN000904 |
| Instrument Name: | Agilent 5975B |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
