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MB Sample ID: SA032613
| Local Sample ID: | 1906046 |
| Subject ID: | SU000615 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Genotype Strain: | N/A|6.53kg|6.87kg|6.09kg|7.23kg|6.48kg |
| Gender: | Male| Male| Male| Male| Male| Male |
| Animal Animal Supplier: | none|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center| |Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/11|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle |
| Animal Inclusion Criteria: | Weight |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000615 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Genotype Strain: | N/A|6.53kg|6.87kg|6.09kg|7.23kg|6.48kg |
| Gender: | Male| Male| Male| Male| Male| Male |
| Animal Animal Supplier: | none|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center| |Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/11|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle |
| Animal Inclusion Criteria: | Weight |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 1906046 | SA032613 | FL007273 | Time Point 3 | Collection Type |
Collection:
| Collection ID: | CO000609 |
| Collection Summary: | Time Point 3 Post-Rx: Observations on NHP response six days after administration of Pyrimethamine|Time Point 4 Rx: Pyrimethamine was administered at 1 mg/kg/day IM for three days|Time Point 5 Post-Rx: Observations on NHP response seven days after initiation of the anti-malarial regimen|Time Point 6 Rx: Pyrimethamine was administered at 1 mg/kg/day IM for three days|Time Point 7 Post-Rx: Observations on NHP response eight days after initiation of the anti-malarial regimen |
| Sample Type: | Whole blood |
| Collection Method: | Intravenous |
| Collection Frequency: | once |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000629 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000622 |
| Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx |
| Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
| Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Anion Exchange Standards: Cystine, cysteine-glutathione disulfide, carboxymethyl-cysteine, glutathione disulfide, carboxymethyl-glutathione. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |
Combined analysis:
| Analysis ID | AN000907 | AN000908 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Ion exchange |
| Chromatography system | Thermo Accela CTC | Thermo Accela CTC |
| Column | Higgins C18 (100 x 2.1mm,5um) | Hamilton Anion Exchange (100 x 2.1mm,7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | unspecified | unspecified |
Chromatography:
| Chromatography ID: | CH000644 |
| Chromatography Summary: | untargeted chromatography (C18 column) |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Higgins C18 (100 x 2.1mm,5um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 350 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Caffeine, Diethyl-toluamide |
| Internal Standard Mt: | 1.5 min; 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000645 |
| Chromatography Summary: | anion exchange chromatography |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Hamilton Anion Exchange (100 x 2.1mm,7um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-2mins = 5%, 2-7mins = gradient to 50%, 7-10mins = 50%; B%: 0-10mins = 50%; C%: 0-2mins = 45%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 350 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Cystine; cysteine-glutathione disulfide; carboxymethyl-cysteine (very low); glutathione disulfide (+1 and +2); carboxymethyl-glutathione |
| Internal Standard Mt: | 1-1.5 min; 2-4 min; 3.5-5 min; 7-8 min; 7-8 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Ion exchange |
MS:
| MS ID: | MS000806 |
| Analysis ID: | AN000907 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | technical triplicates for each sample |
| Ion Mode: | POSITIVE |
| Dataformat: | .raw, .cdf |
| Analysis Protocol File: | Metab_Quality_Control_v1_May_17_2013.docx Metabolomics Core - Data Analysis.docx |
| MS ID: | MS000807 |
| Analysis ID: | AN000908 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | technical triplicates for each sample |
| Ion Mode: | POSITIVE |
| Dataformat: | .raw, .cdf |
| Analysis Protocol File: | Metab_Quality_Control_v1_May_17_2013.docx Metabolomics Core - Data Analysis.docx |
