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MB Sample ID: SA033109
| Local Sample ID: | 9991200102 |
| Subject ID: | SU000622 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Weight Or Weight Range: | NA|6.94kg|9.04kg|8.03kg|8.83kg|8.68kg |
| Gender: | Male|Male|Male|Male|Male|Male |
| Animal Animal Supplier: | Yerkes Field Station|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center||Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/12|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle |
| Animal Inclusion Criteria: | Weight|Weight|Weight|Weight|Weight|Weight |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000622 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Weight Or Weight Range: | NA|6.94kg|9.04kg|8.03kg|8.83kg|8.68kg |
| Gender: | Male|Male|Male|Male|Male|Male |
| Animal Animal Supplier: | Yerkes Field Station|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center||Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/12|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle |
| Animal Inclusion Criteria: | Weight|Weight|Weight|Weight|Weight|Weight |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 9991200102 | SA033109 | FL007355 | N/A | Gender |
| 9991200102 | SA033109 | FL007355 | N/A | Prior Infection |
| 9991200102 | SA033109 | FL007355 | N/A | Days since Inoculation |
| 9991200102 | SA033109 | FL007355 | QC Sample, in-lab prepared | Collection Type |
Collection:
| Collection ID: | CO000616 |
| Collection Summary: | Every Other Day Collection | Time Point 1 Baseline: Uninfected control for each Non-Human Primate (NHP). | Time Point 2 Peak of infection: Peak of infection determined by clinical and parasitological assessment for each NHP. The time point is intended to characterize the host immune response and capture multi-omics data during the peak of parasitemias when the NHP is experiencing clinical signs of disease that are commonly severe in P. coatneyi infections. NHPs were monitored based on clinical and hematological parameters, and clinical interventions with artemether were performed as necessary. | Time Point 3 Post-peak: Observations 7 days after peak infection and sub-curative treatment. | Time Point 4 Recrudescence Peak: This time point tested expectations of a drop in hemoglobin levels and increase in parasitemias due to recrudescence from a chronic infection. After sample collection, NHPs received sub-curative treatment with artemether based on clinical and hematological parameters. | Time Point 5 Post-peak: Chronic infection with low levels of persistent parasitemia that may be below detectable levels. | Time Point 6 Recrudescence Peak: Same goal as time point 4 for 2nd second recrudescence peak. | Time Point 7 Post-peak: Observations of NHP response after a second recrudescence in a chronic phase with low levels of persistent parasitemia. |
| Sample Type: | Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood |
| Collection Method: | Ear Prick|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous |
| Collection Frequency: | Every Other Day|Once|Once|Once|Once|Once|Once|Once |
| Blood Serum Or Plasma: | Plasma|Plasma|Plasma|Plasma|Plasma|Plasma|Plasma|Plasma |
Treatment:
| Treatment ID: | TR000636 |
| Treatment Summary: | Plasmodium Coatneyi Hackeri Strain Sporozoites Inoculation |
| Treatment: | Biotic |
| Treatment Route: | Injection |
Sample Preparation:
| Sampleprep ID: | SP000629 |
| Sampleprep Summary: | aliquots of 30ul sample were mixed with 100ul acetonitrile and 2.5ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 100ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Sampleprep Protocol Filename: | E03_MB_Sample_Preparations_Plasma_serum_HFQE_V1_3282016.docx, E4_E3_E18_E23_E24_E25_MalariaCore_Metabolomics SOP_v1_6October2016.docx |
| Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
| Sample Spiking: | C18 Standards: Caffeine, Diethyl-toluamide. Internal Standards/Stable Isotopes: [13C6]-D-glucose, [15N]-indole, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine |
Combined analysis:
| Analysis ID | AN000917 | AN000918 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Ion Intensity | Ion Intensity |
Chromatography:
| Chromatography ID: | CH000654 |
| Chromatography Summary: | C18 120k resolution method with C18 positive and negative wash |
| Methods Filename: | Metab_HFQE_Setup.docx |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A: 0-2mins = 80%, 2-7mins = gradient to 0%, 7-10mins = 0%. B: 0-2mins = 15%, 2-7mins = gradient to 95%, 7-10mins = 95%. C: 0-10mins = 5% |
| Flow Rate: | 350μL/min |
| Internal Standard: | Cystine; cysteine-glutathione disulfide; carboxymethyl-cysteine (very low); glutathione disulfide (+1 and +2); carboxymethyl-glutathione |
| Internal Standard Mt: | 1.5 min, 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | HESI probe with S-lens combination for ESI |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000816 |
| Analysis ID: | AN000917 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Technical triplicates for each sample |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Capillary Voltage: | 4.45 |
| Collision Gas: | N2 |
| Gas Pressure: | 50 psi |
| Ionization: | electrospray ionization |
| Mass Accuracy: | 5ppm |
| Source Temperature: | 48 |
| Spray Voltage: | 3.5 |
| Atom Gun Current: | 1000000 |
| Dataformat: | .raw, .cdf |
| Probe Tip: | HESI |
| Resolution Setting: | 120000 |
| Scan Range Moverz: | 85-1275 |
| MS ID: | MS000817 |
| Analysis ID: | AN000918 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 300 |
| Capillary Voltage: | 4.45 |
| Collision Gas: | N2 |
| Gas Pressure: | 50 psi |
| Ionization: | electrospray ionization |
| Mass Accuracy: | 5ppm |
| Source Temperature: | 48 |
| Spray Voltage: | 3.5 |
| Atom Gun Current: | 1000000 |
| Dataformat: | .raw, .cdf |
| Probe Tip: | HESI |
| Resolution Setting: | 120000 |
| Scan Range Moverz: | 85-1275 |
