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MB Sample ID: SA033420

Local Sample ID:CCMB09WB
Subject ID:SU000628
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:Healthy - Mean (±SD): 54.9±8.2 years | Sepsis - Mean (+SD): 54.7±17.3 years
Weight Or Weight Range:Healthy - Mean (+SD): 74.4±1.71 kg; BMI < 30
Height Or Height Range:Healthy - Mean (±SD): 171.8±10.3cm
Gender:Healthy - males and females | Sepsis - males and females
Human Race:Healthy - Caucasian (C) and African American (AA) | Sepsis - Caucasian (C) and African American (AA)
Human Medications:Healthy - None
Human Prescription Otc:Healthy - None
Human Smoking Status:Healthy - Non smokers
Human Alcohol Drug Use:Healthy - None
Human Inclusion Criteria:Healthy - non-smoking, non-obese with no known medical conditions that require chronic drug therapy | Sepsis - admitted to emergency department or intensive care unit. Clinical indices for severe sepsis or septic shock were met: confirmed or suspected infection, two or more criteria of systemic inflammatory response criteria, and hypoperfusion evidenced by either a systolic blood pressure of <90mmHg after 20 min of fluid resuscitation or a blood lactate level of at least 36 mg/dL.
Species Group:Human

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Subject:

Subject ID:SU000628
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:Healthy - Mean (±SD): 54.9±8.2 years | Sepsis - Mean (+SD): 54.7±17.3 years
Weight Or Weight Range:Healthy - Mean (+SD): 74.4±1.71 kg; BMI < 30
Height Or Height Range:Healthy - Mean (±SD): 171.8±10.3cm
Gender:Healthy - males and females | Sepsis - males and females
Human Race:Healthy - Caucasian (C) and African American (AA) | Sepsis - Caucasian (C) and African American (AA)
Human Medications:Healthy - None
Human Prescription Otc:Healthy - None
Human Smoking Status:Healthy - Non smokers
Human Alcohol Drug Use:Healthy - None
Human Inclusion Criteria:Healthy - non-smoking, non-obese with no known medical conditions that require chronic drug therapy | Sepsis - admitted to emergency department or intensive care unit. Clinical indices for severe sepsis or septic shock were met: confirmed or suspected infection, two or more criteria of systemic inflammatory response criteria, and hypoperfusion evidenced by either a systolic blood pressure of <90mmHg after 20 min of fluid resuscitation or a blood lactate level of at least 36 mg/dL.
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
CCMB09WBSA033420FL007384Whole BloodSample Type
CCMB09WBSA033420FL007384FGender
CCMB09WBSA033420FL007384CRace

Collection:

Collection ID:CO000622
Collection Summary:Healthy - Direct venipuncture collection of WB and Serum from healthy controls | Sepsis - Indwelling arterial or venous catheter
Collection Protocol Comments:Healthy - Fasting (12h) blood samples (serum and whole blood) were collected between 8:30-9:30am by single direct venipuncture. | Sepsis - Serum generated via indwelling catheters as part of clinical trial (NCT 00372502)
Sample Type:Healthy - Blood: Whole Blood and Serum | Sepsis - Blood serum
Collection Method:Healthy - Direct venipuncture | Sepsis - indwelling catheters
Collection Location:Healthy - Michigan Clinical Research Unit | Sepsis - University of Mississippi
Collection Frequency:Healthy - 1 | Sepsis - 1
Collection Time:Healthy - Fasting, between 0830-0930 | Sepsis - N/A
Storage Conditions:Healthy - Serum: Initial RT then -80°C following processing (within 2h) Whole blood: Initial 0°C then -80°C following processing (within 10mins) | Sepsis - Initial RT then -80°C following processing (within 2h)
Storage Vials:Healthy - SST: Becton-Dickinson vacutainer Serum Separator tubes. WB: Becton-Dickinson vacutainer Sodium Heparin tubes | Sepsis - SST: Becton-Dickinson vacutainer Serum Separator tubes
Collection Tube Temp:Healthy - RT | Sepsis - RT
Additives:Healthy - Serum: none. Whole blood: Sodium Heparin | Sepsis - none
Blood Serum Or Plasma:Healthy - Whole blood and Serum | Sepsis - Serum

Treatment:

Treatment ID:TR000642
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP000635
Sampleprep Summary:Methanol:cholorform extraction, filtration
Sampleprep Protocol Comments:Lipid fractions saved, stored at -20°C
Processing Method:Methanol:Chloroform Extraction, filtration
Processing Storage Conditions:0-4°C
Extraction Method:Methanol:chloroform extraction
Extract Enrichment:Ultra-filtration to remove residual protein
Extract Cleanup:N/A
Extract Storage:-80°C
Sample Resuspension:500 uL Deuterium Oxide (D2O)
Sample Spiking:DSS-IS2 (Chenomx, Inc)

Analysis:

MB Sample ID:SA033420
Analysis ID:AN000926
Laboratory Name:University of Michigan Biochemical NMR Core Laboratory
Analysis Type:NMR
Acquisition Date:5/29/2013, 3/17/2014, 3/19/2014, 3/20/2014,7/29/2014, 8/28/2014
Acquisition Parameters File:metnoesy
Software Version:VNMRJ 3.2
Operator Name:Michael Finkel, Julie Trexel
Data Format:.fid
Num Factors:6
Num Metabolites:53
Units:uM

NMR:

NMR ID:NM000103
Analysis ID:AN000926
Instrument Name:Agilent 500/54 Premium Shielded VNMRS system
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Standard Concentration:10% (~0.5mM)
Spectrometer Frequency:500 MHz
NMR Probe:ONE-Probe
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Auto shim (gradient shimming), manual when necessary.
Pulse Sequence:1D-NOESY
Water Suppression:saturation at 80 Hz induced field strength
Pulse Width:5.5ms
Offset Frequency:around -178Hz
Chemical Shift Ref Cpd:DSS-d6 (whole blood), Formate (serum)
Temperature:25°C
Baseline Correction Method:manual
Chemical Shift Ref Std:DSS-d6 (whole blood), Formate (serum)
Binned Increment:N/A
Binned Data Protocol File:N
A
Binned Data Excluded Range:N/A
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