Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA036116

Local Sample ID:	173
Subject ID:	SU000663
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000663
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
173	SA036116	FL007661	7/20/11	Date of Study
173	SA036116	FL007661	baseline	Time
173	SA036116	FL007661	Overweight/Obese adolescents 5 week exercise program	Study Group

Collection:

Collection ID:	CO000657
Collection Summary:	Male and female American Indians 11-21 years old are being recruited from the Choctaw Nation Health Service Area of southeast Oklahoma. We use a collaborative approach as recently described by our American Indian Programs Coordinator (12). The primary characteristics of the three study groups are shown in Table 1. Group 1 is obese (Body mass index, BMI, ≥95th percentile for age- and sex-specific norms based on growth charts from the Centers for Disease Control), with pubertal maturation level ≥Tanner Stage 2, family history of T2D, and recent history of low physical activity. Low physical activity, defined as attaining <30 minutes of structured moderate-to-vigorous intensity exercise on 3 days/week over the preceding 3 months, is confirmed through questionnaires and use of an accelerometer. Groups 2 and 3 are both classified as normal weight (BMI greater than 20th and less than 85th percentile) with maturation level ≥Tanner Stage 2. Group 2 will have low physical activity (same as Group 1), while Group 3 will have high physical activity, defined as >30 minutes of structured moderate-to-vigorous intensity exercise on >3 days/week in the preceding 3 months. Additional eligibility criteria are: lack of diabetes or other potentially confounding metabolic disorders, able to safely exercise, willingness to complete the testing and participation schedule, and not on medications known to impact the stated outcomes. A medical history and physical exam is performed to assure suitability for the study during the initial screening.
Sample Type:	Blood

Treatment:

Treatment ID:	TR000677
Treatment Summary:	Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff.

Treatment ID:

TR000677

Treatment Summary:

Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff.

Sample Preparation:

Sampleprep ID:	SP000670
Sampleprep Summary:	Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet.

Sampleprep ID:

SP000670

Sampleprep Summary:

Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet.

Combined analysis:

Analysis ID	AN000972
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Agilent 1290 Infinity
Column	Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type	ESI
MS instrument type	Triple quadrupole
MS instrument name	Agilent 6460 QQQ
Ion Mode	NEGATIVE
Units	uM

Chromatography:

Chromatography ID:	CH000697
Instrument Name:	Agilent 1290 Infinity
Column Name:	Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS000867
Analysis ID:	AN000972
Instrument Name:	Agilent 6460 QQQ
Instrument Type:	Triple quadrupole
MS Type:	ESI
Ion Mode:	NEGATIVE