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MB Sample ID: SA036581
| Local Sample ID: | 5863-15 |
| Subject ID: | SU000672 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000672 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 5863-15 | SA036581 | FL007900 | Sedentary | Time point |
| 5863-15 | SA036581 | FL007900 | SED-Naproxen | Grouping |
Collection:
| Collection ID: | CO000666 |
| Collection Summary: | Blood and Tissue Harvesting: Mice will be euthanized and blood will be taken by cardiac puncture, centrifuged, and the plasma will be stored at -80ºC. The heart, diaphragm, and muscles of both hindlimbs (gastrocnemius, soleus, quadriceps) will be dissected and snap frozen in liquid nitrogen. Tibialis anterior and plantaris muscles from both hindlimbs also will be dissected and one set will be snap frozen in liquid nitrogen while the other set will be mounted in OCT and frozen in isopentane cooled by liquid nitrogen. Cryosections will be used to evaluate disease activity and exercise-induced muscle injury by staining with: (a) hematoxylin and eosin to assess gross morphology, cellular infiltration, and necrosis, (b) anti-F4/80 to label macrophages, and (c) anti-IgG or IgM to label damaged muscle fibers. |
| Sample Type: | Heart tissue |
Treatment:
| Treatment ID: | TR000686 |
| Treatment Summary: | Studies will be performed in 12-16 week old male mdx mice, C57BL10 control mice, and nNOS-/- mice obtained from Jackson Laboratory. All protocols will be approved by the Penn State College of Medicine Institutional Animal Care and Use Committee. Drug Treatment: Mice will be treated with vehicle, naproxcinod (20 mg/kg/day) or equimolar naproxen (12.5mg/kg/day) once a day for 7 consecutive days. To avoid the stress of oral gavage or ip injections in mdx mice, drug or vehicle will be administered in a small volume of peanut butter. Most mice readily consume the dosed peanut butter within 30 min. Terminal experiments will be performed 2 hours after the final dose is consumed. |
Sample Preparation:
| Sampleprep ID: | SP000679 |
| Sampleprep Summary: | mouse gastrocnemius NEFA |
Combined analysis:
| Analysis ID | AN000981 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | NEGATIVE |
| Units | picomoles/mg |
Chromatography:
| Chromatography ID: | CH000706 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000876 |
| Analysis ID: | AN000981 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
