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MB Sample ID: SA045808
| Local Sample ID: | S00021739 |
| Subject ID: | SU000845 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000845 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00021739 | SA045808 | FL009111 | shACADMscr | Transduced with |
Collection:
| Collection ID: | CO000839 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000859 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000852 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001300 | AN001301 | AN001302 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Xbridge BEH C18 (50 x 1mm,3.5um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF | Triple quadrupole |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF | Agilent 6490A QQQ |
| Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
| Units | peak area normalized | peak area normalized | pmol/ug protein |
Chromatography:
| Chromatography ID: | CH000910 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000911 |
| Instrument Name: | Agilent |
| Column Name: | Waters Xbridge BEH C18 (50 x 1mm,3.5um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.250 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001193 |
| Analysis ID: | AN001300 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001194 |
| Analysis ID: | AN001301 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001195 |
| Analysis ID: | AN001302 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A008_-_Acyl_Carnitines.pdf |
