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MB Sample ID: SA051189
| Local Sample ID: | Patient 30 |
| Subject ID: | SU000917 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000917 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Patient 30 | SA051189 | FL009855 | Positive | Malaria Infection Status |
Collection:
| Collection ID: | CO000911 |
| Collection Summary: | Breath collection was performed as previously reported with alterations detailed here (see PMID: 25810441). In brief, ≥ 1 L of exhaled breath was collected in a 3 L SamplePro Flexfilm sample bag (SKC Inc.). Using a set flow pump (ACTI-VOC, Markes International), exactly 1 L of breath was pumped through an inert stainless-steel sorbent tube with a triple bed sorbent composed of Tenax 60/80/ Carbograph 1 60/80/ Carboxen 1003 40/60 (Camsco). Sorbent tubes were stored at -20°C prior to shipment on black ice for later mass spectrometric analysis. |
| Sample Type: | Breath |
Treatment:
| Treatment ID: | TR000931 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000924 |
| Sampleprep Summary: | N/A |
Combined analysis:
| Analysis ID | AN001440 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | raw abundance |
Chromatography:
| Chromatography ID: | CH001011 |
| Chromatography Summary: | All samples were run with a TurboMatrix 650 ATD (Perkin Elmer) connected to a Leco Pegasus 4D GCxGC-TOFMS system. Before analysis, sorbent tubes were brought to room temperature and purged for 5 min with BiP N2 (Airgas) at 60 mL/min. A gaseous standard mixture (20.1 ng fluorobenzene, 18.6 ng toluene-D8, 21.7 ng bromofluorobenzene, 20.3 ng 1,2-dichlorobenzene-D4) was added to each tube by the TurboMatrix 650 immediately prior to analysis. Tubes were desorbed at 270°C, 40 mL/min He flow, with recollection on a secondary Tenax cold trap at 10°C. Analytes were released from the secondary trap by heating to 295°C with 20% transferred to the GC/MS. The GC had a 30 m length x 0.25 mm ID x 0.25 µm film thickness DB-5 column (Agilent). The GC oven was programmed to hold at 40°C for 3 min, ramp 5°C/min to 200°C, then ramp 10°C/min to 250°C, final ramp 25°C/min to 300°C, then hold at 300°C for 3 min. The TOFMS had a sampling frequency of 50 Hz and a mass recording range of 34-400 amu. |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Flow Rate: | 1.2 mL/min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001330 |
| Analysis ID: | AN001440 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
