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MB Sample ID: SA051462
| Local Sample ID: | 22 |
| Subject ID: | SU000920 |
| Subject Type: | Plant |
| Subject Species: | Triticum aestivum |
| Taxonomy ID: | 4565 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000920 |
| Subject Type: | Plant |
| Subject Species: | Triticum aestivum |
| Taxonomy ID: | 4565 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 22 | SA051462 | FL009917 | SONALIKA | Strain |
| 22 | SA051462 | FL009917 | Control | Treatment |
| 22 | SA051462 | FL009917 | 10 | Timepoint |
Collection:
| Collection ID: | CO000914 |
| Collection Summary: | None |
| Sample Type: | Plant |
| Volumeoramount Collected: | Flag leaf |
| Storage Conditions: | -80 C |
Treatment:
| Treatment ID: | TR000934 |
| Treatment: | Abiotic |
| Plant Growth Location: | Gainesville, FL |
| Plant Light Period: | 12H |
| Plant Humidity: | 70% |
| Plant Temp: | Control: 24C/12Hrs, 18C/12Hrs & Stress: Day 36C/12Hrs, Night 28C/12Hrs |
| Plant Watering Regime: | Irrigated |
| Plant Estab Date: | 2016-09-09 |
| Plant Harvest Date: | 2016-12-01 |
| Plant Growth Stage: | Anthesis |
| Plant Harvest Method: | Leaves were washed with distilled water, cut and freeze in liquid nitrogen |
| Plant Storage: | -80C |
Sample Preparation:
| Sampleprep ID: | SP000927 |
| Sampleprep Summary: | Weigh 30mg of the lyophilized and pulverized sample into clean Eppendorf tube and record weight. Add 20μL of Metabolites Internal Standard Mix to each sample. Add 750 μL of Methanol and 750 μL of 10mM Ammonium Acetate to each sample. Homogenize for 30 seconds at 1800 rpm on the BeadBeater. Sonicate for 10-20 minutes at room temperature. Centrifuge at room temperature for 10 minutes at 17,000 xg. Transfer 50 μL of supernatant to a labeled LC vial with a fused insert. Transfer the rest of the supernatant to a labeled 2 mL LC Vial with no fused insert to store in -80°C. |
| Sampleprep Protocol Filename: | Plant_Tissue_SamplePrep_Instrument_method.pdf Appendix_A -_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
| Analysis ID | AN001443 | AN001444 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | Peak Height |
Chromatography:
| Chromatography ID: | CH001014 |
| Methods Filename: | Plant_Tissue.pdf |
| Chromatography Comments: | Reverse phase or HILIC- HPLC-MS |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001333 |
| Analysis ID: | AN001443 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | Plant_Tissue_SamplePrep_Instrument_method.pdf |
| MS ID: | MS001334 |
| Analysis ID: | AN001444 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | Plant_Tissue_SamplePrep_Instrument_method.pdf |
