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MB Sample ID: SA052932
| Local Sample ID: | Beta_9_4 |
| Subject ID: | SU000940 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Weight Or Weight Range: | 180-220 g |
| Gender: | Male |
| Animal Animal Supplier: | Beijing Huafukang Bioscience |
| Animal Light Cycle: | 12:12 light-dark cycle |
| Animal Feed: | Standard food |
| Animal Water: | Free access to food and water |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000940 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Weight Or Weight Range: | 180-220 g |
| Gender: | Male |
| Animal Animal Supplier: | Beijing Huafukang Bioscience |
| Animal Light Cycle: | 12:12 light-dark cycle |
| Animal Feed: | Standard food |
| Animal Water: | Free access to food and water |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Beta_9_4 | SA052932 | FL010018 | CUMS model | Genotype |
| Beta_9_4 | SA052932 | FL010018 | Fluoxetine | Treatment |
Collection:
| Collection ID: | CO000934 |
| Collection Summary: | Male Sprague-Dawley (SD) rats (180-220 g) were randomly assigned to control or CUMS group. Rats in CUMS group were exposed to one random stressor per day for 35 d. Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, Fluoxetine or Albiflroin for 7 days via intragastric gavage. |
| Sample Type: | Plasma |
| Storage Conditions: | -80C |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000954 |
| Treatment Summary: | Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, fluoxetine or albiflorin for 7 days via intragastric gavage |
| Treatment: | Antidepressants |
| Treatment Compound: | Albiflorin or fluoxetine |
| Treatment Route: | Intragastric gavage |
| Treatment Dose: | Albiflorin 7 mg/kg; fluoxetine 10 mg/kg |
| Treatment Dosevolume: | 0.2 ml/g |
| Treatment Doseduration: | 7 days |
| Treatment Vehicle: | Saline |
| Animal Anesthesia: | No |
| Animal Acclimation Duration: | No |
| Animal Fasting: | No |
Sample Preparation:
| Sampleprep ID: | SP000947 |
| Sampleprep Summary: | Briefly, for plasma samples, 95 µl of plasma was mixed with 5 µl of isotope standards mix and extracted with 400 µl of pre-cold extraction buffer containing methanol/acetonitrile (50/50, v/v). The mixture was incubated on ice for 30 min and centrifuged for 10 min at 16,000 g and 4 ºC. The supernatant containing both polar and nonpolar metabolites was transferred to new tubes and stored at -80 ºC for metabolomic analysis |
| Extraction Method: | methanol/acetonitrile (50/50, v/v) precipitation |
| Extract Storage: | -80 ºC |
| Sample Derivatization: | No |
| Sample Spiking: | Isotope standards |
Combined analysis:
| Analysis ID | AN001469 | AN001470 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Waters ACQUITY UPLC | Waters ACQUITY UPLC |
| Column | Luna NH2 aminopropyl HPLC | Luna NH2 aminopropyl HPLC |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | AUC | AUC |
Chromatography:
| Chromatography ID: | CH001033 |
| Instrument Name: | Waters ACQUITY UPLC |
| Column Name: | Luna NH2 aminopropyl HPLC |
| Column Pressure: | 2200 psi |
| Column Temperature: | 25 °C |
| Flow Rate: | 0.35 ml/min |
| Injection Temperature: | 4 °C |
| Sample Injection: | 12 µl |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide; 20 mM ammonium acetate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Sample Loop Size: | 12 |
| Randomization Order: | Yes |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001357 |
| Analysis ID: | AN001469 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Collision Energy: | Compound dependent |
| Collision Gas: | 25 |
| Mass Accuracy: | 0.1 Da |
| Source Temperature: | 475 ºC |
| Spray Voltage: | -4500 ºC |
| MS ID: | MS001358 |
| Analysis ID: | AN001470 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Collision Energy: | Compound dependent |
| Collision Gas: | 25 |
| Mass Accuracy: | 0.1 Da |
| Source Temperature: | 475 ºC |
| Spray Voltage: | 5500 ºC |
