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MB Sample ID: SA053409
| Local Sample ID: | 170607aOEsa28_1 |
| Subject ID: | SU000948 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000948 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 170607aOEsa28_1 | SA053409 | FL010054 | MECFS | Diagnosis |
| 170607aOEsa28_1 | SA053409 | FL010054 | FEMALE | Sex |
Collection:
| Collection ID: | CO000942 |
| Collection Summary: | CSF samples obtained from lumbar punctures were retrieved from bio-repositories at Sierra Internal Medicine (SIM) and Wisconsin Viral Research Group (WVRG). These biobank specimens were collected over time and maintained at -80°C. |
| Sample Type: | CSF |
Treatment:
| Treatment ID: | TR000962 |
| Treatment Summary: | 3 groups: 32 ME/CFS cases (11 men, 21 women) 59 comparator/control subjects (18 men, 41 women), including: 40 subjects with multiple sclerosis (MS) 19 subjects with non-infectious/non-inflammatory conditions (ND) |
Sample Preparation:
| Sampleprep ID: | SP000955 |
| Sampleprep Summary: | 1) Aliquot 20 uL into 2 sample sets one for GC and one for LC analysis 2) Add 1 mL of 3:3:2 extraction solvent to ach tube 3) Vortex 10 sec 4) Shake 5 min 5) Centrifuge at 14000 RCF for 2 min 6) Aliquot 450 uL of supernatant into two sets of tubes saving one set for backup 7) Pipet the rest of sample into falcon tube 8) Dry down samples saving 1 set of backups 9) Aliquot 2 mL of the pooled samples from the falcon tube into 2 mL tubes then centrifuge again 10) Aliquot 450 uL of the pooled samples from the falcon tube into 1.5 mL tubes to dry down 11) Place pooled samples in centrivap to dry |
Combined analysis:
| Analysis ID | AN001480 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus III GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Counts |
Chromatography:
| Chromatography ID: | CH001038 |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
| Chromatography Comments: | Excel generated |
| Instrument Name: | Leco Pegasus III GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 7.7 PSI (initial condition) |
| Column Temperature: | 50 - 330°C |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 50°C ramped to 250°C by 12°C/s |
| Sample Injection: | 0.5µl |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min |
| Transferline Temperature: | 230°C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001364 |
| Analysis ID: | AN001480 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | 70eV |
| Mass Accuracy: | Nominal |
| Source Temperature: | 250°C |
| Scan Range Moverz: | 85-500 |
| Scanning Cycle: | 17 Hz |
| Scanning Range: | 80-500 Da |
| Skimmer Voltage: | 1850 V |
