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MB Sample ID: SA054096

Local Sample ID:CP21
Subject ID:SU000956
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:35 - 65
Weight Or Weight Range:61 - 75
Gender:Female

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Subject:

Subject ID:SU000956
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:35 - 65
Weight Or Weight Range:61 - 75
Gender:Female

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
CP21SA054096FL010095Control patientDisease Status

Collection:

Collection ID:CO000950
Collection Summary:Venous blood samples were taken in the morning after overnight fasting and were collected using K3EDTA tubes
Sample Type:Blood
Collection Method:Venous
Collection Frequency:one
Volumeoramount Collected:5 mL
Storage Conditions:-80C
Additives:K3EDTA
Blood Serum Or Plasma:Plasma

Treatment:

Treatment ID:TR000970
Treatment Summary:None
Human Fasting:Overnight

Sample Preparation:

Sampleprep ID:SP000963
Sampleprep Summary:40 µL of plasma was deproteinized with cold acetonitrile (1:3,−20 °C), followed by a two-step derivatization: (i) methoximation with O-Methoxyamine hydrochloride in pyridine (15 mg/mL, room temperature, 16 h) followed by (ii) silylation with BSTFA containing 1% TMCS (70 °C, 1 h).
Processing Method:Protein precipitation followed by metabolite extraction
Processing Storage Conditions:On ice
Extraction Method:Acetonitrile
Sample Derivatization:Methoximation and silylation

Combined analysis:

Analysis ID AN001503 AN001504 AN001505
Analysis type MS MS MS
Chromatography type GC Reversed phase Reversed phase
Chromatography system Agilent Agilent 1200 Agilent 1200
Column Zebron ZB-5MSi capillary GC Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) Phenomenex Kinetex C18 (150 x 2.1mm,2.6um)
MS Type EI ESI ESI
MS instrument type Single quadrupole QTOF QTOF
MS instrument name Agilent 5973 Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE POSITIVE NEGATIVE
Units peak area peak area peak area

Chromatography:

Chromatography ID:CH001060
Chromatography Summary:Untargeted GC/MS method
Instrument Name:Agilent
Column Name:Zebron ZB-5MSi capillary GC
Column Temperature:0-1 min 60ªC 1-33 min to 320ªC
Flow Rate:1.0 mL/min
Sample Injection:2 uL
Chromatography Type:GC
  
Chromatography ID:CH001061
Chromatography Summary:Untargeted LC/MS method
Chromatography Comments:HPLC system 1200 series coupled to Q-TOF 6520
Instrument Name:Agilent 1200
Column Name:Phenomenex Kinetex C18 (150 x 2.1mm,2.6um)
Column Pressure:300 bar
Column Temperature:40°C
Flow Gradient:25% B increased then to 95% B in 35 min, returned to initial conditions in 1 min and was kept constant for 9 min to ensure re-equilibration of the column
Flow Rate:0.3 mL/min
Sample Injection:10 uL
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS001386
Analysis ID:AN001503
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Helium Flow:1.0 mL/min
Ion Source Temperature:150 C
Ionization:Electron ionization
Ionization Energy:70 eV
Source Temperature:230 ºC
Scan Range Moverz:50 - 600
Scanning:1.38 scan/s
  
MS ID:MS001387
Analysis ID:AN001504
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500 V
Scan Range Moverz:100 / 1200 m/z
Scanning:1.38 scan/s
  
MS ID:MS001388
Analysis ID:AN001505
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:4000 V
Scan Range Moverz:100 / 1200 m/z
Scanning:1.38 scan/s
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