Return to study ST000939 main page
MB Sample ID: SA055887
| Local Sample ID: | S_6_B |
| Subject ID: | SU000978 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000978 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S_6_B | SA055887 | FL010215 | Steroid Sensitive | Treatment Response |
| S_6_B | SA055887 | FL010215 | M | Gender |
Collection:
| Collection ID: | CO000972 |
| Collection Summary: | None |
| Collection Protocol Comments: | Citrate plasma |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR000992 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000985 |
| Sampleprep Summary: | Study sample aliquots of 180 µL were mixed with 60 µL 0.9% saline solution containing 0.2% NaN3 and 4 mM formate in D2O. A total pool was also created by combining 11 µL aliquots from each study sample, mixed thoroughly, divided into four 180 µL aliquots, and processed identically to study samples. All of the samples were thoroughly mixed on multiple tube vortex mixer for 30 s at 5000 rpm, centrifuged at room temperature and 12,000 rcf for 5 min. The supernatants (200 µL) were stored in a fresh 1.5 mL centrifuge tubes overnight at 4 C, before transfer into pre-labeled 3mm NMR tubes (Bruker-Biospin, Switzerland) on the day of analysis. |
| Extract Storage: | 4℃ |
Analysis:
| MB Sample ID: | SA055887 |
| Analysis ID: | AN001540 |
| Laboratory Name: | David H. Murdock Research Institute at Kannapolis, NC |
| Analysis Type: | NMR |
| Software Version: | Bruker TopSpin 3.2 |
| Operator Name: | Kevin Knagge / Jessica Gooding |
| Detector Type: | NMR |
| Data Format: | 1r, fid |
| Num Factors: | 7 |
| Num Metabolites: | 28 |
| Units: | mM in NMR Sample |
NMR:
| NMR ID: | NM000119 |
| Analysis ID: | AN001540 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 1 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | CRYO QNP |
| NMR Solvent: | D2O |
| NMR Tube Size: | 3 mm |
| Shimming Method: | topshim |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | Yes |
| Pulse Width: | 12.781 us |
| Power Level: | 19 W |
| Receiver Gain: | 4 |
| Offset Frequency: | 3294.20 Hz |
| Chemical Shift Ref Cpd: | formate |
| Temperature: | 25 |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.892 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 12 ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | Formate |
| Binned Increment: | 0.04 |
| Binned Data Excluded Range: | water (4.55 – 5.15 ppm) and citrate (2.47-2.565 and 2.61-2.70) |
