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MB Sample ID: SA056019
| Local Sample ID: | S00027020 |
| Subject ID: | SU000983 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000983 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00027020 | SA056019 | FL010240 | Blinded | SampleType |
Collection:
| Collection ID: | CO000977 |
| Collection Summary: | None |
| Sample Type: | Plasma;Feces;Cells;Tissue |
Treatment:
| Treatment ID: | TR000997 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000990 |
| Sampleprep Summary: | Amino acids are analyzed using the Phenomenex EZfaast kit. Samples are extracted, semi-purified, derivitized and measured by EI-GCMS using norvaline as an internal standard for normalization. See reference for User Guide and analytical details. Analytes are reported as uM with CV's generally 10%, but Histidine is exceptional with a CV ~30 |
| Sampleprep Protocol Filename: | Amino_acids_analysis.pdf |
Combined analysis:
| Analysis ID | AN001549 | AN001550 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | Reversed phase |
| Chromatography system | Agilent 7890N | Agilent 6490A |
| Column | Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | EI | ESI |
| MS instrument type | Single quadrupole | Triple quadrupole |
| MS instrument name | Agilent | Agilent 6490A QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | uM | uM |
Chromatography:
| Chromatography ID: | CH001087 |
| Chromatography Summary: | None |
| Methods Filename: | EZGCMS_1.M |
| Instrument Name: | Agilent 7890N |
| Column Name: | Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex) |
| Chromatography Type: | GC |
| Chromatography ID: | CH001088 |
| Methods Filename: | QM-004-HSS_50mm_ACar+_MRM-Insert-gradtest3 |
| Instrument Name: | Agilent 6490A |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001428 |
| Analysis ID: | AN001549 |
| Instrument Name: | Agilent |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001429 |
| Analysis ID: | AN001550 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
