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MB Sample ID: SA057118
Local Sample ID: | 20150818_T_memory_plate9 |
Subject ID: | SU000990 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000990 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20150818_T_memory_plate9 | SA057118 | FL010284 | CD8_T_memory | cell_type |
Collection:
Collection ID: | CO000984 |
Collection Summary: | Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate. |
Collection Protocol ID: | blood_collection cell_quench_collection cells_media |
Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
Sample Type: | T-Cells |
Treatment:
Treatment ID: | TR001004 |
Treatment Summary: | Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin. |
Treatment Protocol ID: | normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc |
Sample Preparation:
Sampleprep ID: | SP000997 |
Sampleprep Summary: | Cells of interest extracted from the blood. Polar extraction from cells. |
Sampleprep Protocol ID: | cell_extraction polar_extraction |
Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
Combined analysis:
Analysis ID | AN001560 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | GC-EI-MS, Thermo ITQ |
Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
MS Type | EI |
MS instrument type | GC-ITQ |
MS instrument name | Thermo ITQ |
Ion Mode | POSITIVE |
Units | normalized peak area |
Chromatography:
Chromatography ID: | CH001094 |
Chromatography Summary: | Targeted GC |
Instrument Name: | GC-EI-MS, Thermo ITQ |
Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
Chromatography Type: | GC |
MS:
MS ID: | MS001438 |
Analysis ID: | AN001560 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
Ion Mode: | POSITIVE |