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MB Sample ID: SA057196
| Local Sample ID: | 13765079 |
| Subject ID: | SU000993 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 11-67 yo |
| Weight Or Weight Range: | 32-194 kg |
| Height Or Height Range: | 141-388 cm |
| Gender: | Male and female |
| Human Race: | 47% white, 53% african american |
| Human Ethnicity: | 95% not Hispanic |
| Human Trial Type: | Open-label, prospective cohort study |
| Human Lifestyle Factors: | NA |
| Human Medications: | fluticasone (was not the intervention in this project, all participants received this drug for 2 weeks prior to sample collection) |
| Human Prescription Otc: | prescription |
| Human Smoking Status: | 9% current, 20% former |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000993 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 11-67 yo |
| Weight Or Weight Range: | 32-194 kg |
| Height Or Height Range: | 141-388 cm |
| Gender: | Male and female |
| Human Race: | 47% white, 53% african american |
| Human Ethnicity: | 95% not Hispanic |
| Human Trial Type: | Open-label, prospective cohort study |
| Human Lifestyle Factors: | NA |
| Human Medications: | fluticasone (was not the intervention in this project, all participants received this drug for 2 weeks prior to sample collection) |
| Human Prescription Otc: | prescription |
| Human Smoking Status: | 9% current, 20% former |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 13765079 | SA057196 | FL010310 | 2 | race |
| 13765079 | SA057196 | FL010310 | 2 | diplotype |
| 13765079 | SA057196 | FL010310 | 2 | ethnicity |
| 13765079 | SA057196 | FL010310 | 2 | ever_smoked |
| 13765079 | SA057196 | FL010310 | 2 | daily_dose |
Collection:
| Collection ID: | CO000987 |
| Collection Summary: | Whole blood is collected in an EDTA vacutainer at Visit 2 after two weeks of fluticasone therapy. 1 ml aliquots at 80°C |
| Sample Type: | Blood (whole) |
| Collection Method: | Whole blood is collected in an EDTA vacutainer (Visit 2; after two weeks of fluticasone therapy) and is stored on ice. Within 2 hr of collection, the sample is centrifuged at 2000 rpm in a 4°C refrigerated benchtop centrifuge and the plasma is recovered and stored in aliquots at -80°C. |
| Collection Location: | Nemours Children's Clinic Phlebotomy Laboratory. |
| Collection Frequency: | One time |
| Storage Conditions: | Described in summary |
| Collection Vials: | 10 ml potassium EDTA vacutainer |
| Storage Vials: | 1.4 ml Micronic unthreaded 2D data matrix tubes with TPE push caps |
| Collection Tube Temp: | 4°C |
| Additives: | potassium EDTA |
Treatment:
| Treatment ID: | TR001007 |
| Treatment Summary: | All subjects will be treated with at least 110 mg of fluticasone propionate once or twice daily (Flovent® MDI) during the 2-week treatment period. |
| Treatment Protocol ID: | NCT00708227 |
| Treatment Protocol Comments: | Note: Fluticasone was not the intervention in this project; all study participants received this drug for 2 weeks prior to blood collection for the sample being used for the metabolomic analysis. However, participants did receive different doses of fluticasone in order to have them remain on the same dose that they were on prior to study entry. This was to ensure that their asthma remained stable at the end of the 2 week treatment with fluticasone. |
| Treatment Compound: | fluticasone |
| Treatment Route: | nebulizer; inhaled |
| Treatment Dose: | 110 mcg, 220 mcg, 440 mcg, 880 mcg |
| Treatment Doseduration: | daily |
Sample Preparation:
| Sampleprep ID: | SP001000 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
| Analysis ID | AN001564 | AN001565 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | Peak height |
Chromatography:
| Chromatography ID: | CH001097 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001442 |
| Analysis ID: | AN001564 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001443 |
| Analysis ID: | AN001565 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
