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MB Sample ID: SA057512
| Local Sample ID: | ms6707-14 |
| Subject ID: | SU001000 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001000 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms6707-14 | SA057512 | FL010357 | Group 4 | Group |
Collection:
| Collection ID: | CO000994 |
| Collection Summary: | Samples were collected by cardiac puncture and plasma was collected following standard centrifugation steps. Immediately following the samples were separated into 500 uL aliquots in 600 uL tubes and frozen in liquid nitrogen. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001014 |
| Treatment Summary: | We administered normal, high glucose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis (Group 1-4 samples). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group (Group 9). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group with antibiotic (Group 10). For the gut microbiota experiment, during the last 10 days of the diet subsets of all groups will have bacitracin and streptomycin (B/S) provided in their drinking water (0.5g/250 mL). The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process. Group1 = normal diet Group2 = high glucose diet Group3 = aspartame diet Group4 = acesulfame potassium diet Group5 = rat gut microbiota normal diet + antibotics Group6 = rat gut microbiota high glucose diet + antibotics Group7 = rat gut microbiota aspartame diet + antibotics Group8 = rat gut acesulfame potassium diet + antibotics Group9 = fructose diet Group10 = rat gut fructose diet + antibotics |
Sample Preparation:
| Sampleprep ID: | SP001007 |
| Sampleprep Summary: | Concentration of AA + metabolites in rat plasma |
Combined analysis:
| Analysis ID | AN001575 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH001104 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001453 |
| Analysis ID: | AN001575 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
