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MB Sample ID: SA060000
| Local Sample ID: | 160719cOEsa31_1 |
| Subject ID: | SU001020 |
| Subject Type: | Other |
| Subject Species: | Felis catus |
| Taxonomy ID: | 9685 |
| Age Or Age Range: | 5-10 years |
| Weight Or Weight Range: | 3.3-6.2 kg |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001020 |
| Subject Type: | Other |
| Subject Species: | Felis catus |
| Taxonomy ID: | 9685 |
| Age Or Age Range: | 5-10 years |
| Weight Or Weight Range: | 3.3-6.2 kg |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 160719cOEsa31_1 | SA060000 | FL010554 | Placebo | Group |
| 160719cOEsa31_1 | SA060000 | FL010554 | Baseline (days 5-7) | Timepoint |
| 160719cOEsa31_1 | SA060000 | FL010554 | Male castrated | Gender |
| 160719cOEsa31_1 | SA060000 | FL010554 | 9 | Age at start of study (years) |
Collection:
| Collection ID: | CO001014 |
| Collection Summary: | First-morning naturally-voided feces were collected daily on the last 3 days of baseline (days 5-7), treatment (26-28), and recovery (68-70) |
| Sample Type: | Feces |
| Collection Method: | Natural voiding |
| Collection Frequency: | Daily |
| Collection Duration: | 3 days of each time period |
| Volumeoramount Collected: | 1 gm |
| Storage Conditions: | -80℃ |
| Collection Vials: | 2 mL screw cap polypropylene vials |
| Storage Vials: | 2 mL screw cap polypropylene vials |
| Collection Tube Temp: | Room temperature |
| Additives: | None |
Treatment:
| Treatment ID: | TR001034 |
| Treatment Summary: | The placebo treatment group was administered Clindamycin orally with a placebo once daily for 21 days with reevaluation 603 days thereafter. The synbiotic treatment group was administered Clindamycin orally with a commercially-available synbiotic (Proviable DC) once daily for 21 days with reevaluation 603 days thereafter. |
| Treatment Compound: | Clindamycin, placebo (Placebo treatment group); Clindamycin, Proviable DC (Synbiotic treatment group) |
| Treatment Route: | PO |
| Treatment Dose: | 75 mg, 2 capsules |
| Treatment Dosevolume: | 21 days |
Sample Preparation:
| Sampleprep ID: | SP001027 |
| Sampleprep Summary: | Samples were immediately frozen and remained in storage at -80ºC pending completion of data collection. Samples for each cat from each timepoint were combined directly prior to sample analysis to generate pooled samples for analysis. Genomic DNA was extracted from 100 mg of feces from each pooled sample using a commercially available kit (PowerSoil®, Mo Bio, Carlsbad, CA USA) according to manufacturer’s protocol. 10 mg of lyophilized feces from each pooled sample were used for metabolomic analyses. |
| Processing Method: | Lyophilization |
Combined analysis:
| Analysis ID | AN001607 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Counts |
Chromatography:
| Chromatography ID: | CH001129 |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 7.7 PSI (initial condition) |
| Column Temperature: | 50 - 330°C |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 50°C ramped to 250°C by 12°C/s |
| Sample Injection: | 0.5 uL |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min |
| Transferline Temperature: | 230°C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Randomization Order: | Excel generated |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001485 |
| Analysis ID: | AN001607 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | 70eV |
| Mass Accuracy: | Nominal |
| Scan Range Moverz: | 85-500 |
| Scanning Cycle: | 17 Hz |
| Scanning Range: | 80-500 Da |
| Skimmer Voltage: | 1850 |
