Return to study ST000998 main page
MB Sample ID: SA061756
| Local Sample ID: | ms6724-30 |
| Subject ID: | SU001037 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001037 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms6724-30 | SA061756 | FL010685 | DC | Grouping |
Collection:
| Collection ID: | CO001031 |
| Collection Summary: | After obtaining informed consent, portal and peripheral venous blood (4 ml of each) and bile (2 mL) was collected intraoperatively in a red-top tube by a Mayo Clinic LT surgeon. Blood was placed on ice, promptly fractionated by centrifugation, divided into 100 μL aliquots, and stored at -80°C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001051 |
| Treatment Summary: | We prospectively enrolled three groups of participants from the Mayo Clinic Liver Transplant inpatient service and outpatient clinics and have collected samples from: i) 9 patients with PSC who underwent living- donor LT, ii) 15 donors (normal controls), and iii) 20 patients with cirrhosis due to a disorder other than PSC who underwent LT (disease controls). The following inclusion and exclusion criteria were applied: Inclusion criteria 1. Adult (age>18 years). 2. PSC patient undergoing LT, healthy living donor, or other chronic liver disease patient undergoing LT. Exclusion Criteria: 1. Females who are pregnant or attempting to become pregnant. 2. Concomitant liver disease (e.g. chronic viral hepatitis in addition to PSC). 3. Acute intestinal disease (infectious enterocolitis, IBD flare) in the past 6 months. 4. Treatment with any investigational drugs within the past 6 months. 5. Use of antibiotics within the past 4 weeks. 6. Any previous organ transplant. 7. Hemodialysis. |
Sample Preparation:
| Sampleprep ID: | SP001044 |
| Sampleprep Summary: | bile acid concentrations |
Combined analysis:
| Analysis ID | AN001627 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Cohesive TX2 |
| Column | Altma HP HILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH001145 |
| Instrument Name: | Cohesive TX2 |
| Column Name: | Altma HP HILIC (150 x 2.1mm,5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001503 |
| Analysis ID: | AN001627 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | uM |
| Ion Mode: | POSITIVE |
