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MB Sample ID: SA062153
| Local Sample ID: | WN092_b1_4 |
| Subject ID: | SU001038 |
| Subject Type: | Other |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Age Or Age Range: | young adult |
| Gender: | Hermaphrodite |
| Species Group: | Recombinant inbred lines |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001038 |
| Subject Type: | Other |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Age Or Age Range: | young adult |
| Gender: | Hermaphrodite |
| Species Group: | Recombinant inbred lines |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WN092_b1_4 | SA062153 | FL010690 | RIL strain | Strain type |
Collection:
| Collection ID: | CO001032 |
| Collection Summary: | A synchronous population of young adult worms was washed off the plates in M9 buffer and the worm pellet was washed with dH2O for three times and then collected in a 2 mL Eppendorf tube and freeze-dried overnight. Dried worm pellets were stored at room temperature until use. |
| Sample Type: | Worms |
| Storage Conditions: | Room temperature |
Treatment:
| Treatment ID: | TR001052 |
| Treatment Summary: | Nematodes were cultured and maintained at 20°C on nematode growth media (NGM) agar plates. Culture conditions in all experiments were the same unless indicated otherwise. For metabolite profiling of 199 RIL strains, N2, and CB4856, age synchronized worms were obtained by alkaline hypochlorite treatment of gravid adults grown on E. coli OP50 lawn, 2000 eggs of each strain were then seeded onto NGM plates and cultured for 2.5 days allowing development to young adults. |
Sample Preparation:
| Sampleprep ID: | SP001045 |
| Sampleprep Summary: | A synchronous population of young adult worms was washed off the plates in M9 buffer and the worm pellet was washed with dH2O for three times and then collected in a 2 mL Eppendorf tube and freeze-dried overnight. Dried worm pellets were stored at room temperature until use. A dry worm pellet was re-suspended in ice-cold 0.9% NaCl solution (250 µL). Worms were homogenized with a 5 mm steel bead using a TissueLyser II (Qiagen) for 2x2.5 min at frequency of 30 times/sec, followed by a tip sonication (energy level: 40 joule; output: 8 watts) for two times on ice water. Protein quantification was performed with BCA assay. |
Combined analysis:
| Analysis ID | AN001628 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Waters Acquity |
| Column | none |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Quattro Premier XE |
| Ion Mode | NEGATIVE |
| Units | nmol/mg of protein |
Chromatography:
| Chromatography ID: | CH001146 |
| Chromatography Summary: | For fatty acids:The MS system consisted of an Acquity UPLC Binary Solvent manager (Waters, Milford MA) and an Acquity UPLC sample manager connected to a Quattro Premier XE mass spectrometer (Waters, Milford MA), used in the negative ESI mode. For amino acids:Liquid chromatography was performed at 50°C using a Acquity UPLC BEH C18, 1.7 µm, 2.1 x 100 mm column (Waters, Milford MA) and the injected volume was 10 µL. Mass spectrometry experiments were performed using a Micromass Quattro Premier XE Tandem Mass Spectrometer (waters, Milford, MA). The mass spectrometer was used in the multiple reaction monitoring mode (MRM) in the ESI-positive mode. |
| Instrument Name: | Waters Acquity |
| Column Name: | none |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS001504 |
| Analysis ID: | AN001628 |
| Instrument Name: | Waters Quattro Premier XE |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
