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MB Sample ID: SA069219
| Local Sample ID: | 170731dngsa21_2.cdf |
| Subject ID: | SU001073 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001073 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 170731dngsa21_2.cdf | SA069219 | FL011045 | medium only | treatment |
Collection:
| Collection ID: | CO001067 |
| Collection Summary: | Samples were 0.2 uM filtered and frozen in liquid nitrogen. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001087 |
| Treatment Summary: | Arabidopsis T87 suspension cells were treated with the pathogen-associated molecular pattern elf26 or a DMSO-control treatment for six hours, then incubated in fresh media for one hour. The conditioned medium (exudate) was collected from each culture by centrifugation and 0.22 um filter to remove plant cells. |
Sample Preparation:
| Sampleprep ID: | SP001080 |
| Sampleprep Summary: | 100 µL of each exudate sample was concentrated to dryness in a Centrivap cold trap vacuum concentrator (Labconco). Sample derivatization, chromatographic parameters, instrument parameters and data processing methods were as described [Fiehn et al. (2008) Quality control for plant metabolomics: reporting MSI-compliant studies. Plant J. 53:691–704]. |
Combined analysis:
| Analysis ID | AN001695 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | POSITIVE |
| Units | peak heights |
Chromatography:
| Chromatography ID: | CH001194 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001570 |
| Analysis ID: | AN001695 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
