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MB Sample ID: SA071405
| Local Sample ID: | LS_S12555 |
| Subject ID: | SU001093 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001093 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LS_S12555 | SA071405 | FL011202 | Low Salt | Diet |
Collection:
| Collection ID: | CO001087 |
| Collection Summary: | Blood was collected from rats after completing either the high salt or low salt diet for a number of days. Rats fasted overnight before blood collection. Blood was then processed to submit only the plasma for GC-MS analysis. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001107 |
| Treatment Summary: | 18 rats were placed into either a high salt diet group (2.0% NaCl) or a low salt diet group (0.3% NaCl), to create 2 groups of 9 rats each. The diet began at day 32 of life and continued for a number of days. |
Sample Preparation:
| Sampleprep ID: | SP001100 |
| Sampleprep Summary: | Samples were vortexed for 3 seconds to homogenize. Added 30 µL of each sample to a 1.5 mL eppendorf tube. Added 1 mL of extraction solvent (3:3:2 ACN:IPA:Water). Vortexed for 10 seconds. Shake for 6 minutes at 4°C. Centrifuged for 2 minutes at 14000 rcf. Aliquoted 2 x 475 µL portions of the supernatant into new tubes. Save one aliquote at -20°C for backup and dry the other in a centrivap to complete dryness. After the sample is dry, add 500 µL of 50:50 ACN:IPA. Centifuged for 2 minutes at 14000 rcf. Aliquoted 475 µL of supernatant to a new tube. Evaporate to complete dryness in centrivap. Submit for derivitzation. |
Combined analysis:
| Analysis ID | AN001720 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001216 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 50-330C |
| Flow Rate: | 1 mL min^(-1) |
| Injection Temperature: | 50°C ramped to 250°C by 12°C s-1 |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001592 |
| Analysis ID: | AN001720 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
