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MB Sample ID: SA072352
| Local Sample ID: | Amb_R3_T1 |
| Subject ID: | SU001115 |
| Subject Type: | Plant |
| Subject Species: | Panicum maximum(Megathyrsis maximus (Jacq.)) |
| Taxonomy ID: | 59788 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001115 |
| Subject Type: | Plant |
| Subject Species: | Panicum maximum(Megathyrsis maximus (Jacq.)) |
| Taxonomy ID: | 59788 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Amb_R3_T1 | SA072352 | FL011322 | Control- ambient temperature and ambient [CO2] | Treatment |
| Amb_R3_T1 | SA072352 | FL011322 | 3 | Ring |
| Amb_R3_T1 | SA072352 | FL011322 | 30 | Time Point (days) |
Collection:
| Collection ID: | CO001109 |
| Collection Summary: | Leaf material dried and processed as stated Ulanov et al. (2010) J of Plant Phys |
| Sample Type: | Plant |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001129 |
| Treatment Summary: | Ambient(Amb) : Control plots with ambient [CO2] and ambient temperature elvTemp(T): elevated temperature plots (+2C) and ambient [CO2] elvCO2(eC): elevated [CO2] plots (600ppm) and ambient temperature elvTemp+elvCO2(eCeT): elevated temperature (+2C) and elevated [CO2] (600ppm). Time Point A(2014-05-22) and Time Point B(2014-07-14) |
| Treatment Protocol Comments: | Time Point A(2014-05-22) and Time Point B(2014-07-14) |
| Treatment: | elevated [CO2] and/or elevated temperature |
| Treatment Doseduration: | 30 days and 50days |
| Plant Growth Location: | MiniFACE facility at University of São Paul in Ribeirão Preto, São Paulo Brazil |
| Plant Plot Design: | Complete replicated design (n=4) |
| Plant Light Period: | ambient |
| Plant Harvest Date: | 2014-05-22 |
| Plant Metab Quench Method: | Immersion in liquid N2 within 1 min of harvest |
| Plant Harvest Method: | Youngest fully expanded leaves was cut (PO:0001053), placed in foil, quenched in LN2 |
| Plant Storage: | -80 C, then lyophilized and ground, shipped on dry ice to University of Illinois at Urbana-Champagin were stored in 20 C in falcon tubes |
Sample Preparation:
| Sampleprep ID: | SP001122 |
| Sampleprep Summary: | Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample |
| Sampleprep Protocol Comments: | As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431. |
| Processing Method: | Homogenization and Solvent removal with speed vac |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | 1:1 MeOH/H20 |
| Extract Storage: | -80℃ |
| Sample Spiking: | Internal Standard (hentriacontanoic acid 5 uL) |
Combined analysis:
| Analysis ID | AN001753 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Phenomenea ZB-5MS (60m x 0.32mm,0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001237 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Phenomenea ZB-5MS (60m x 0.32mm,0.25um) |
| Column Pressure: | 19.223 psi |
| Flow Rate: | 2.4ml/min |
| Injection Temperature: | 250C |
| Internal Standard: | C31:0 fatty acid (hentriacontanoate) |
| Sample Injection: | split (5:1), 1ul |
| Analytical Time: | 65 min |
| Oven Temperature: | 70C - 310C |
| Running Voltage: | 1094 emvolts |
| Transferline Temperature: | 250 |
| Strong Wash Solvent Name: | pyridine |
| Strong Wash Volume: | 10ul |
| Sample Syringe Size: | 10ul |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001621 |
| Analysis ID: | AN001753 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Helium Flow: | 2.4 |
| Ion Source Temperature: | 230 |
| Ionization Energy: | 69.9 |
| Mass Accuracy: | low |
| Scan Range Moverz: | 50-800 |
| Scanning Cycle: | 4 |
