Return to study ST001071 main page
MB Sample ID: SA072353
Local Sample ID: | Amb_R3_T2 |
Subject ID: | SU001115 |
Subject Type: | Plant |
Subject Species: | Panicum maximum(Megathyrsis maximus (Jacq.)) |
Taxonomy ID: | 59788 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001115 |
Subject Type: | Plant |
Subject Species: | Panicum maximum(Megathyrsis maximus (Jacq.)) |
Taxonomy ID: | 59788 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Amb_R3_T2 | SA072353 | FL011323 | Control- ambient temperature and ambient [CO2] | Treatment |
Amb_R3_T2 | SA072353 | FL011323 | 3 | Ring |
Amb_R3_T2 | SA072353 | FL011323 | 50 | Time Point (days) |
Collection:
Collection ID: | CO001109 |
Collection Summary: | Leaf material dried and processed as stated Ulanov et al. (2010) J of Plant Phys |
Sample Type: | Plant |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001129 |
Treatment Summary: | Ambient(Amb) : Control plots with ambient [CO2] and ambient temperature elvTemp(T): elevated temperature plots (+2C) and ambient [CO2] elvCO2(eC): elevated [CO2] plots (600ppm) and ambient temperature elvTemp+elvCO2(eCeT): elevated temperature (+2C) and elevated [CO2] (600ppm). Time Point A(2014-05-22) and Time Point B(2014-07-14) |
Treatment Protocol Comments: | Time Point A(2014-05-22) and Time Point B(2014-07-14) |
Treatment: | elevated [CO2] and/or elevated temperature |
Treatment Doseduration: | 30 days and 50days |
Plant Growth Location: | MiniFACE facility at University of São Paul in Ribeirão Preto, São Paulo Brazil |
Plant Plot Design: | Complete replicated design (n=4) |
Plant Light Period: | ambient |
Plant Harvest Date: | 2014-05-22 |
Plant Metab Quench Method: | Immersion in liquid N2 within 1 min of harvest |
Plant Harvest Method: | Youngest fully expanded leaves was cut (PO:0001053), placed in foil, quenched in LN2 |
Plant Storage: | -80 C, then lyophilized and ground, shipped on dry ice to University of Illinois at Urbana-Champagin were stored in 20 C in falcon tubes |
Sample Preparation:
Sampleprep ID: | SP001122 |
Sampleprep Summary: | Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample |
Sampleprep Protocol Comments: | As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431. |
Processing Method: | Homogenization and Solvent removal with speed vac |
Processing Storage Conditions: | Room temperature |
Extraction Method: | 1:1 MeOH/H20 |
Extract Storage: | -80℃ |
Sample Spiking: | Internal Standard (hentriacontanoic acid 5 uL) |
Combined analysis:
Analysis ID | AN001753 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Phenomenea ZB-5MS (60m x 0.32mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975C |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH001237 |
Instrument Name: | Agilent 7890A |
Column Name: | Phenomenea ZB-5MS (60m x 0.32mm,0.25um) |
Column Pressure: | 19.223 psi |
Flow Rate: | 2.4ml/min |
Injection Temperature: | 250C |
Internal Standard: | C31:0 fatty acid (hentriacontanoate) |
Sample Injection: | split (5:1), 1ul |
Analytical Time: | 65 min |
Oven Temperature: | 70C - 310C |
Running Voltage: | 1094 emvolts |
Transferline Temperature: | 250 |
Strong Wash Solvent Name: | pyridine |
Strong Wash Volume: | 10ul |
Sample Syringe Size: | 10ul |
Chromatography Type: | GC |
MS:
MS ID: | MS001621 |
Analysis ID: | AN001753 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Helium Flow: | 2.4 |
Ion Source Temperature: | 230 |
Ionization Energy: | 69.9 |
Mass Accuracy: | low |
Scan Range Moverz: | 50-800 |
Scanning Cycle: | 4 |