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MB Sample ID: SA072471
| Local Sample ID: | ATQ-20170224 |
| Subject ID: | SU001118 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | 3D7 |
| Cell Counts: | 1x10^8 per technical replicate |
| Species Group: | Plasmodium falciparum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001118 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | 3D7 |
| Cell Counts: | 1x10^8 per technical replicate |
| Species Group: | Plasmodium falciparum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ATQ-20170224 | SA072471 | FL011359 | Atovaquone | Drug |
| ATQ-20170224 | SA072471 | FL011359 | 10 nM | Treatment conc. |
Collection:
| Collection ID: | CO001112 |
| Collection Summary: | Aspiration of 4 mL media, transfer of sample (1 mL containing infected red blood cells) to 1.5 mL tubes |
| Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
| Sample Type: | Parasite |
| Collection Method: | Aspiration of 4 mL media, transfer of sample (1 mL) to 1.5 mL tubes |
| Collection Location: | Biological safety hood |
| Collection Frequency: | once after 2.5 hours treatment |
| Collection Duration: | Collection and extraction occurs in under 20 minutes per plate |
| Volumeoramount Collected: | 1 mL containing 1x10^8 infected cells |
| Storage Conditions: | On ice |
| Collection Vials: | 1.5 mL eppendorf |
| Storage Vials: | 1.5 mL eppendorf |
Treatment:
| Treatment ID: | TR001132 |
| Treatment Summary: | Infected Red Blood cells were exposed to Antimalarial Compounds, associated with sample names |
| Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
| Treatment: | Drug Treatment |
| Treatment Compound: | MMV1432165, MMV1451822, MMV1068987, MMV1490405, MMV1490397, MMV1449124, MMV1432711, MMV1454442, MMV1010248, MMV1319550, MMV1427995, MMV1431916, MMV011772 |
| Treatment Route: | Addition to media |
| Treatment Dose: | 10xIC50 |
| Treatment Dosevolume: | 10xIC50 |
| Treatment Doseduration: | 2.5 hours |
| Treatment Vehicle: | Stocks kept in either DMSO or Water |
| Cell Storage: | Temperature/Gas Mixture controlled incubator |
| Cell Growth Container: | 6-well plate |
| Cell Growth Config: | 5 mL per well, each with 1x10^8 cells |
| Cell Media: | RPMI 1640 w/ Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
| Cell Harvesting: | PBS Wash followed by 90% methanol extraction |
Sample Preparation:
| Sampleprep ID: | SP001125 |
| Sampleprep Summary: | Samples washed in PBS, spun down, aspirated, extracted in 90% methanol with labelled aspartate, then dried, resuspended in water labelled with chlorpropamide |
| Sampleprep Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
| Processing Method: | Wash, Lysis, Drying, resuspension |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 90% Methanol Lysis |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 100 uL water with chlorpropamide |
Combined analysis:
| Analysis ID | AN001756 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | Average Peak Area |
Chromatography:
| Chromatography ID: | CH001240 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001624 |
| Analysis ID: | AN001756 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
