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MB Sample ID: SA072839
| Local Sample ID: | PCC 7002 240 SEC REP 2 |
| Subject ID: | SU001124 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus sp. PCC 7002 |
| Taxonomy ID: | 32049 |
| Genotype Strain: | Synechococcus |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001124 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus sp. PCC 7002 |
| Taxonomy ID: | 32049 |
| Genotype Strain: | Synechococcus |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PCC 7002 240 SEC REP 2 | SA072839 | FL011385 | Wild type | Genotype |
Collection:
| Collection ID: | CO001118 |
| Collection Summary: | The experiment was conducted in a reactor at a metabolic steady state (OD 730 nm = 0.8). 13C labeled sodium bicarbonate was used as a tracer. Tracer concentration was 1 g/L. After the introduction of the tracer, 15 mL of the culture were collected at time point : 0, 60, 120, 180 and 240 secs. Samples were quenched with methanol and extracted using methanol-chloroform-water method. Extracts were stored at -80 degree C till LCMS analysis. LCMS analysis was done in negative ion mode using information dependent aquisition (IDA) method. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001138 |
| Treatment Summary: | The metabolites were extracted using a methanol-chloroform-water method described in the Metabolite Extraction Protocol_PCC 7002 file of the collection data. |
Sample Preparation:
| Sampleprep ID: | SP001131 |
| Sampleprep Summary: | The metabolite extracts of different collected samples were reconstituted in 100 μL of 50/50 methanol-water mixture and filtered before injecting on LC/MS. Injection volume was 10 μL. |
Combined analysis:
| Analysis ID | AN001763 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001246 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
| Flow Gradient: | 0% B (0 min), 0% B (2min), 35% B (8 min), 35% B (10.5 min), 90% B (15.50 min), 90% B (20.5 min) and 0% B (22 min) |
| Flow Rate: | 0.3 mL/minute |
| Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001630 |
| Analysis ID: | AN001763 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
