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MB Sample ID: SA074837
| Local Sample ID: | V029 |
| Subject ID: | SU001141 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 30-60 years old |
| Weight Or Weight Range: | 52.3-176.6 kg |
| Gender: | Male and female |
| Human Medications: | To be included in the study all participants had to be taking an atypical (second generation) antipsychotic |
| Human Inclusion Criteria: | DSM IV diagnosis of a schizophrenia spectrum diagnosis who had been taking an atypical antipsychotic for at least 6 months, with no changes in antipsychotic regimen for 8 weeks preceding the baseline visit. Twenty age and sex matched obese and nonobese participants without mental health diagnoses were included to match the BMI range of the patients with schizophrenia as BMI controls. |
| Human Exclusion Criteria: | Current use of insulin, diagnosis of diabetes mellitus type 2 prior to antipsychotic exposure, active substance abuse diagnosis |
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Subject:
| Subject ID: | SU001141 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 30-60 years old |
| Weight Or Weight Range: | 52.3-176.6 kg |
| Gender: | Male and female |
| Human Medications: | To be included in the study all participants had to be taking an atypical (second generation) antipsychotic |
| Human Inclusion Criteria: | DSM IV diagnosis of a schizophrenia spectrum diagnosis who had been taking an atypical antipsychotic for at least 6 months, with no changes in antipsychotic regimen for 8 weeks preceding the baseline visit. Twenty age and sex matched obese and nonobese participants without mental health diagnoses were included to match the BMI range of the patients with schizophrenia as BMI controls. |
| Human Exclusion Criteria: | Current use of insulin, diagnosis of diabetes mellitus type 2 prior to antipsychotic exposure, active substance abuse diagnosis |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| V029 | SA074837 | FL011566 | Schizophrenia dx | Treatment |
Collection:
| Collection ID: | CO001135 |
| Collection Summary: | Fasting blood samples were collected via venipuncture into an additive-free vial and allowed to coagulate at room temperature for 30 minutes. They were then centrifuged for 15 minutes at 2500 rcf to obtain serum. Samples were frozen at -80C, thawed once for sample processing related to parent study, then kept at -80C until processing for NMR and TLC-GC assays. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
| Collection Vials: | Additive-free vacutainer |
Treatment:
| Treatment ID: | TR001155 |
| Treatment Summary: | N/A: this was an observational study |
Sample Preparation:
| Sampleprep ID: | SP001148 |
| Sampleprep Summary: | Serum samples were subjected to a 1:1 methanol:chloroform extraction, then lyophilized. Prior to NMR the aqueous fraction samples were resuspended and filtered. Prior to fatty acid analysis the lipid fractions were resuspended and purified by TLC prior to GC analysis. This is described in more detail in the attached document. |
| Sampleprep Protocol ID: | EllingrodSamplePrepProtocol |
| Processing Method: | methanol:chloroform extraction, filtration |
| Processing Storage Conditions: | 4? |
| Extraction Method: | methanol:chloroform |
| Extract Enrichment: | ultra-filtration (3kDa filters) to remove residual protein |
| Extract Cleanup: | N/A |
| Extract Storage: | -80? |
| Sample Resuspension: | 500uL deuterium oxide for aqueous fraction, lipid fraction was resuspended in hexane prior to GC analysis |
| Sample Spiking: | aqueous fraction: CaFormate 12mM, lipid faction: C17:0 methyl ester |
Analysis:
| MB Sample ID: | SA074837 |
| Analysis ID: | AN001785 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
| Num Metabolites: | 38 |
| Units: | uM |
NMR:
| NMR ID: | NM000135 |
| Analysis ID: | AN001785 |
| Instrument Name: | Agilent 500/54 Premium Shielded |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | ONE-Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming) |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Offset Frequency: | around -178Hz |
| Chemical Shift Ref Cpd: | Formate |
| Temperature: | 25 |
| Number Of Scans: | 32 |
| Baseline Correction Method: | manual |
| Chemical Shift Ref Std: | Calcium formate |
