Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA075057

Local Sample ID:	J
Subject ID:	SU001145
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Subject:

Subject ID:	SU001145
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
J	SA075057	FL011590	Burned	Burn Status
J	SA075057	FL011590	Yes	Infected

Collection:

Collection ID:	CO001139
Collection Summary:	Blood were aseptically collected from mice directly from the heart then left at room temperature for a few hours to clot, then centrifuged to separate the serum. Serum was then packed on dry ice and sent to the WCMC center in Davis, CA.
Sample Type:	Serum

Treatment:

Treatment ID:	TR001159
Treatment Summary:	Mice were placed into 1 of 4 treatment groups, with three mice in each group. The first group contained uninjured mice who were not infected. The second group contained thermally injured mice who were not infected. The third group contained mice that were uninjured and infected with Pseudomonas aeruginosa. The fourth group of mice were thermally injured and infected with Pseudomonas aeruginosa.

Sample Preparation:

Sampleprep ID:	SP001152
Sampleprep Summary:	30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis.

Sampleprep ID:

SP001152

Sampleprep Summary:

30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis.

Combined analysis:

Analysis ID	AN001790
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890A
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC-TOF
MS instrument name	Leco Pegasus III GC TOF
Ion Mode	POSITIVE
Units	Peak Height

Chromatography:

Chromatography ID:	CH001264
Instrument Name:	Agilent 7890A
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Temperature:	50-330°C
Flow Rate:	1 mL min^(-1)
Injection Temperature:	50°C ramped to 250°C by 12°C s-1
Oven Temperature:	50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min
Chromatography Type:	GC

MS:

MS ID:	MS001652
Analysis ID:	AN001790
Instrument Name:	Leco Pegasus III GC TOF
Instrument Type:	GC-TOF
MS Type:	EI
Ion Mode:	POSITIVE