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MB Sample ID: SA075406

Local Sample ID:M_767_125
Subject ID:SU001153
Subject Type:Mammal
Subject Species:Ovis aries
Taxonomy ID:9940
Age Or Age Range:Gestational days 125-birth

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Subject:

Subject ID:SU001153
Subject Type:Mammal
Subject Species:Ovis aries
Taxonomy ID:9940
Age Or Age Range:Gestational days 125-birth

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
M_767_125SA075406FL011650ControlTreatment
M_767_125SA075406FL011650MaternalSource

Collection:

Collection ID:CO001147
Collection Summary:Blood samples were collected from ewe and fetus on gestational days 125, 130, 135, 138, 140, and every subsequent day until birth (control: 144±1; CORT: 142±2 days gestational age);
Sample Type:Blood (serum)
Collection Method:samples for metabolomics analysis were collected in glass tubes on ice with no anticoagulant and spun for 20 min at 4 °C; the resulting serum was stored at -20 °C until metabolomic analysis.
Collection Location:University of Florida
Storage Conditions:-20℃
Collection Vials:Eppendorf tubes
Storage Vials:Eppendorf tubes

Treatment:

Treatment ID:TR001167
Treatment Summary:The treatment protocol for this study was previous published in Antolic, A., et al. (2018). "Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth." Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352.
Treatment Compound:Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA)
Treatment Dose:1 mg/kg/day

Sample Preparation:

Sampleprep ID:SP001160
Sampleprep Summary:Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).

Analysis:

MB Sample ID:SA075406
Analysis ID:AN001802
Laboratory Name:Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida
Analysis Type:NMR
Analysis Protocol File:Serum_CORT_raw_data_cpmg.zip
Acquisition Parameters File:Serum_CORT_raw_data_cpmg.zip
Software Version:Topspin 3.6
Operator Name:Jacquelyn Walejko
Processing Parameters File:Serum_CORT_raw_data_cpmg.zip
Num Factors:4
Num Metabolites:38
Units:Area under curve

NMR:

NMR ID:NM000138
Analysis ID:AN001802
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:H2O+D2O
Spectrometer Frequency:600 MHz
NMR Probe:5mm Cryoprobe
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Topshim
Pulse Sequence:cpmgpr1d
Water Suppression:Presaturation
Power Level:7.37 W
Receiver Gain:64
Offset Frequency:2824.2
Presaturation Power Level:6.41E-05
Temperature:4C
Number Of Scans:128
Dummy Scans:16
Acquisition Time:1.8173
Relaxation Delay:4 sec
Spectral Width:15.0182 ppm
Num Data Points Acquired:32764
Real Data Points:65536
Line Broadening:1
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