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MB Sample ID: SA075730
| Local Sample ID: | Heads Zt22 - Rep2_HFD |
| Subject ID: | SU001155 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001155 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Heads Zt22 - Rep2_HFD | SA075730 | FL011660 | Head Tissue | Organ |
| Heads Zt22 - Rep2_HFD | SA075730 | FL011660 | Cornmeal Drosphila Diet + 15% coconut Oil | Treatment |
Collection:
| Collection ID: | CO001149 |
| Collection Summary: | After each subject completed the treatment for the required time, the head of the fly was removed from the body over dry ice. Each portion of tissue was placed in a 1.5 mL tube and stored at -80°C until shipped to the WCMC for analysis. |
| Sample Type: | Tissue |
| Volumeoramount Collected: | Variable, depending on the size of the fly. |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 mL |
| Storage Vials: | 1.5 mL |
| Collection Tube Temp: | Dry Ice, -78.5°C |
Treatment:
| Treatment ID: | TR001169 |
| Treatment Summary: | 48 flies were split into two treatment groups, with 24 flies in each group. Group 1 consisted of a High Fat Diet (HFD, Cornmeal diet + 15% coconut oil) and Group 2 consisted of a Non-Fat Diet (NFD, Cornmeal). Within each group, flies were placed in one of four smaller groups, depending on how long they would be on either the HFD or NFD. These groups are ZT4, ZT10, ZT16, ZT22. Each of these smaller groups consisted of 6 flies, to create 4 groups of 6 flies each in both of the diet treatments. |
Sample Preparation:
| Sampleprep ID: | SP001162 |
| Sampleprep Summary: | Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_blood-GCTOF-11082012_332.pdf |
| Sampleprep Protocol Comments: | Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3. |
| Sample Derivatization: | Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C. |
Combined analysis:
| Analysis ID | AN001804 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001271 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001662 |
| Analysis ID: | AN001804 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | -70 eV |
| Dataformat: | .CDF |
| Resolution Setting: | 17 spectra s-1 |
