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MB Sample ID: SA079368
Local Sample ID: | 8784 |
Subject ID: | SU001207 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001207 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
8784 | SA079368 | FL012188 | Control | Diagnosis |
Collection:
Collection ID: | CO001201 |
Collection Summary: | PRISM subject stool samples were collected at the MGH gastroenterology clinic and stored at -80°C prior to DNA extraction. For the Netherlands validation cohort subjects enrolled collected stool at home and then froze it within 15 min in a conventional freezer. A research nurse visited all participants at home to collect home-frozen stool samples, which were then transported and stored at -80°C. The stool samples were kept frozen at -80°C prior to DNA extraction or metabolomic profiling. |
Sample Type: | Stool |
Storage Conditions: | Described in summary |
Treatment:
Treatment ID: | TR001222 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP001215 |
Sampleprep Summary: | Stool samples (weight range 50-5167.8 mg) were homogenized in 4 µL of water per milligram stool sample weight using a bead mill (TissueLyser II; Qiagen) and the aqueous homogenates were aliquoted for metabolite profiling analyses. |
Combined analysis:
Analysis ID | AN001878 | AN001879 | AN001880 | AN001881 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
Column | Waters 150 x 2 mm Atlantis HILIC | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Waters 150 x 2 mm ACQUITY T3 | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
Units | abundance | abundance | abundance | abundance |
Chromatography:
Chromatography ID: | CH001359 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters 150 x 2 mm Atlantis HILIC |
Chromatography Type: | HILIC |
Chromatography ID: | CH001360 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Chromatography Type: | HILIC |
Chromatography ID: | CH001361 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters 150 x 2 mm ACQUITY T3 |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001362 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001734 |
Analysis ID: | AN001878 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Detailed acquisition and data processing methods available in Fransoza et al. (2019) DOI 10.1038/s41564-018-0306-4 |
Ion Mode: | POSITIVE |
MS ID: | MS001735 |
Analysis ID: | AN001879 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Detailed acquisition and data processing methods available in Fransoza et al. (2019) DOI 10.1038/s41564-018-0306-4 |
Ion Mode: | NEGATIVE |
MS ID: | MS001736 |
Analysis ID: | AN001880 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Detailed acquisition and data processing methods available in Fransoza et al. (2019) DOI 10.1038/s41564-018-0306-4 |
Ion Mode: | NEGATIVE |
MS ID: | MS001737 |
Analysis ID: | AN001881 |
Instrument Name: | Thermo Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Detailed acquisition and data processing methods available in Fransoza et al. (2019) DOI 10.1038/s41564-018-0306-4 |
Ion Mode: | POSITIVE |