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MB Sample ID: SA080040
Local Sample ID: | A3 |
Subject ID: | SU001218 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001218 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
A3 | SA080040 | FL012261 | Ang II | Infused |
Collection:
Collection ID: | CO001212 |
Collection Summary: | Approximately 1 mL mouse blood was collected with EDTA-contained tube and and stored at −80°C before further analysis. |
Sample Type: | Blood (whole) |
Treatment:
Treatment ID: | TR001233 |
Treatment Summary: | WT mice were subcutaneously infused with Angiotensin II or saline by mini-pump. |
Sample Preparation:
Sampleprep ID: | SP001226 |
Sampleprep Summary: | The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
Combined analysis:
Analysis ID | AN001903 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo-finnegan DSQ |
Column | Restek 5% phenyl |
MS Type | EI |
MS instrument type | GC/MS |
MS instrument name | Thermo DSQ |
Ion Mode | UNSPECIFIED |
Units | AU |
Chromatography:
Chromatography ID: | CH001378 |
Chromatography Summary: | The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp is from 40° to 300° C in a 16 minute period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below. |
Instrument Name: | Thermo-finnegan DSQ |
Column Name: | Restek 5% phenyl |
Chromatography Type: | GC |
MS:
MS ID: | MS001759 |
Analysis ID: | AN001903 |
Instrument Name: | Thermo DSQ |
Instrument Type: | GC/MS |
MS Type: | EI |
MS Comments: | MS type: Single Quadrupole Ion Mode: Electron Impact Comments: Metabolon Proprietary software used for data analysis and compound annotation |
Ion Mode: | UNSPECIFIED |