Return to study ST001153 main page
MB Sample ID: SA080048
| Local Sample ID: | C5 |
| Subject ID: | SU001218 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001218 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C5 | SA080048 | FL012262 | Saline | Infused |
Collection:
| Collection ID: | CO001212 |
| Collection Summary: | Approximately 1 mL mouse blood was collected with EDTA-contained tube and and stored at −80°C before further analysis. |
| Sample Type: | Blood (whole) |
Treatment:
| Treatment ID: | TR001233 |
| Treatment Summary: | WT mice were subcutaneously infused with Angiotensin II or saline by mini-pump. |
Sample Preparation:
| Sampleprep ID: | SP001226 |
| Sampleprep Summary: | The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
Combined analysis:
| Analysis ID | AN001903 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo-finnegan DSQ |
| Column | Restek 5% phenyl |
| MS Type | EI |
| MS instrument type | GC/MS |
| MS instrument name | Thermo DSQ |
| Ion Mode | UNSPECIFIED |
| Units | AU |
Chromatography:
| Chromatography ID: | CH001378 |
| Chromatography Summary: | The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp is from 40° to 300° C in a 16 minute period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. The information output from the raw data files was automatically extracted as discussed below. |
| Instrument Name: | Thermo-finnegan DSQ |
| Column Name: | Restek 5% phenyl |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001759 |
| Analysis ID: | AN001903 |
| Instrument Name: | Thermo DSQ |
| Instrument Type: | GC/MS |
| MS Type: | EI |
| MS Comments: | MS type: Single Quadrupole Ion Mode: Electron Impact Comments: Metabolon Proprietary software used for data analysis and compound annotation |
| Ion Mode: | UNSPECIFIED |
