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MB Sample ID: SA080496
| Local Sample ID: | U41_A_3b |
| Subject ID: | SU001228 |
| Subject Type: | Other |
| Subject Species: | Acropora cervicornis |
| Taxonomy ID: | 6130 |
| Genotype Strain: | U25, U41, U44 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001228 |
| Subject Type: | Other |
| Subject Species: | Acropora cervicornis |
| Taxonomy ID: | 6130 |
| Genotype Strain: | U25, U41, U44 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| U41_A_3b | SA080496 | FL012329 | U41 | Genotype |
Collection:
| Collection ID: | CO001222 |
| Collection Summary: | Coral colonies were brought to the surface intact, and ~3 cm nubbins were clipped from actively growing branch tips. Nubbins were placed in 20 mL scintillation vials containing 10 mL of 100% methanol spiked with a 0.005 mM aminoanthracene standard and immedately placed on ice. Samples were stored in a -20°C freezer overnight, then transported back to the laboratory n ice and again stored in a 20°C freezer overnight prior to extraction. The next day, samples were agitated for 5 minutes, then allowed to settle for one hour in the -20°C freezer. One mL of the sample extract was transferred to clean 1.5 mL microcentrifuge tubes and centrifuged at 20,000 g for 5 minutes. The supernatant was then transferred to a new 1.5 mL microcentrifuge tube and stored in a -80°C freezer until processing. |
| Sample Type: | Tissue and skeleton |
Treatment:
| Treatment ID: | TR001243 |
| Treatment Summary: | No treatment was applied; study was conducted on natural metabolomic variation among genotypes |
Sample Preparation:
| Sampleprep ID: | SP001236 |
| Sampleprep Summary: | Corol holobiont extract (in methonol) was added to double distilled water (1:2 v/v of sample to water), then flash freeze lyophilized (Labconco) until dry. Lyophilized dry powder was re-suspended in phosphate buffer in deuterium oxide at pH 7. The final volume for the 1H-NMR samples was 60 μL (in a 1.5 mm O.D. tube) with 90 % (v/v) of deuterated 50 mM sodium phosphate buffer (pH 7) with 2 mM of ethylene diamine tetra-acetic acid (EDTA). The remaining 10 % (v/v) was occupied by an internal standard [5 mM D6-4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS-D6) and 0.2% sodium azide in deuterated environment; Chenomx, Inc.]. |
| Processing Method: | Lyophilization |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN001923 | AN001924 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | Peak Height |
Chromatography:
| Chromatography ID: | CH001396 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001779 |
| Analysis ID: | AN001923 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metaboanalyst for data processing |
| Ion Mode: | POSITIVE |
| MS ID: | MS001780 |
| Analysis ID: | AN001924 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metaboanalyst for data processing |
| Ion Mode: | NEGATIVE |
