Return to study ST001179 main page
MB Sample ID: SA081606
| Local Sample ID: | Old-3 |
| Subject ID: | SU001245 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wild type |
| Age Or Age Range: | 8-week-old and 28-month-old |
| Weight Or Weight Range: | 25.4 ± 0.2 g for young and 32.0 ± 0.2 g for aged |
| Gender: | Male |
| Animal Animal Supplier: | Shimizu Laboratory Supplies (Kyoto, Japan) |
| Animal Light Cycle: | 12-h light-dark cycle |
| Animal Feed: | CE2 |
| Species Group: | C57BL/6J |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001245 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wild type |
| Age Or Age Range: | 8-week-old and 28-month-old |
| Weight Or Weight Range: | 25.4 ± 0.2 g for young and 32.0 ± 0.2 g for aged |
| Gender: | Male |
| Animal Animal Supplier: | Shimizu Laboratory Supplies (Kyoto, Japan) |
| Animal Light Cycle: | 12-h light-dark cycle |
| Animal Feed: | CE2 |
| Species Group: | C57BL/6J |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Old-3 | SA081606 | FL012448 | 28m | Age |
Collection:
| Collection ID: | CO001239 |
| Collection Summary: | Gastrocnemius muscles were collected and flash-frozen in liquid N2. |
| Sample Type: | Muscle |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001260 |
| Treatment Summary: | No specific treatment to mention. |
Sample Preparation:
| Sampleprep ID: | SP001253 |
| Sampleprep Summary: | Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment. |
Combined analysis:
| Analysis ID | AN001956 | AN001957 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | fold | fold |
Chromatography:
| Chromatography ID: | CH001418 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS001811 |
| Analysis ID: | AN001956 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS001812 |
| Analysis ID: | AN001957 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
