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MB Sample ID: SA081616
| Local Sample ID: | PCC 11802 1 PERCENT CO2 R3 |
| Subject ID: | SU001246 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus elongatus PCC 11802 |
| Taxonomy ID: | 2283154 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001246 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus elongatus PCC 11802 |
| Taxonomy ID: | 2283154 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PCC 11802 1 PERCENT CO2 R3 | SA081616 | FL012450 | 1% CO2 | Conditions |
Collection:
| Collection ID: | CO001240 |
| Collection Summary: | The PCC 11802 was grown under ambient and 1% CO2 conditions in triplicates in shake flasks at 200 uE light intensity, 38 degrees C temperature, 120 rpm. The samples were collected in exponential phase (OD730 = 0.6).The metabolites were extracted using methanol-chloroform-water method as described in Metabolite Extraction Protocol file. The samples were stored in a -80 degrees C deep freezer until analyzed using LCMS. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001261 |
| Treatment Summary: | The metabolites were extracted using a methanol-chloroform-water method described in the Metabolite Extraction file of the collection data. |
Sample Preparation:
| Sampleprep ID: | SP001254 |
| Sampleprep Summary: | The metabolite extracts of different collected samples were reconstituted in 100 μL of 50/50 methanol-water mixture. An equal amount of fully 13C-labeled biomass extract from cyanobacterium Synechococcus elongatus PCC 11801 was added as internal standard. The mixture is vortexed. The samples are filtered through syringe filters. Injection volume was 6 uL. |
Combined analysis:
| Analysis ID | AN001958 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Area Ratio |
Chromatography:
| Chromatography ID: | CH001419 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
| Flow Gradient: | 0% B (0 min), 0% B (2min), 35% B (8 min), 35% B (10.5 min), 90% B (15.50 min), 90% B (20.5 min) and 0% B (22 min) |
| Flow Rate: | 0.3 mL/minute |
| Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001813 |
| Analysis ID: | AN001958 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data Acquisition was done using IDA method with MS/MS trigerred for top 10 abundant precursor ions. Cycle time = 1s |
| Ion Mode: | NEGATIVE |
| Collision Energy: | -30 |
| Ion Source Temperature: | 450 |
