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MB Sample ID: SA082497
| Local Sample ID: | 30h_Control_n=3_1 |
| Subject ID: | SU001255 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001255 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 30h_Control_n=3_1 | SA082497 | FL012546 | Control | Treatment |
| 30h_Control_n=3_1 | SA082497 | FL012546 | 30 | Time (hr) |
Collection:
| Collection ID: | CO001249 |
| Collection Summary: | 1x108 Cells per technical replicate were aliquoted into an individual microcentrifuge tube, centrifuged, washed once with 1mL ice-cold PBS, centrifuged, and metabolites extracted with 150uL 80% acetonitrile |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001270 |
| Treatment Summary: | Prior to metabolite extraction, P. falciparum-infected human erythrocyte cultures were treated/untreated with indolmycin |
Sample Preparation:
| Sampleprep ID: | SP001263 |
| Sampleprep Summary: | Following metabolite extraction with 80% acetontrile. Samples were centrifuged at top speed for 5 minutes and the supernatant collected. Metabolite samples were then stored at -80 until ready for LC-MS analysis |
Combined analysis:
| Analysis ID | AN001980 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 6550 |
| Column | SeQuant ZIC-HILIC (150 x 4.6mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE |
| Units | ion count |
Chromatography:
| Chromatography ID: | CH001429 |
| Methods Filename: | pHILIC 45 minute |
| Instrument Name: | Agilent 6550 |
| Column Name: | SeQuant ZIC-HILIC (150 x 4.6mm,3.5um) |
| Column Pressure: | 50-150 bar |
| Column Temperature: | 35 |
| Flow Gradient: | 80% |
| Flow Rate: | 0.3 mL/minute |
| Injection Temperature: | 4 |
| Solvent A: | 100% water; 20 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001834 |
| Analysis ID: | AN001980 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Full m/z scan range across 2-35 minutes. Extended dynamic range, 0-1700 m/z |
| Ion Mode: | NEGATIVE |
