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MB Sample ID: SA082598

Local Sample ID:A_cc_4
Subject ID:SU001256
Subject Type:Fungi
Subject Species:Ophiocordyceps sinensis;Cordyceps militaris
Taxonomy ID:72228;73501

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Subject:

Subject ID:SU001256
Subject Type:Fungi
Subject Species:Ophiocordyceps sinensis;Cordyceps militaris
Taxonomy ID:72228;73501

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
A_cc_4SA082598FL012559Ophiocordyceps sinensisSpecies
A_cc_4SA082598FL012559Boiled waterTreatment

Collection:

Collection ID:CO001250
Collection Summary:two species were collected: Ophiocordyceps sinensis and Cordyceps militaris
Sample Type:fungi

Treatment:

Treatment ID:TR001271
Treatment Summary:two treatments: boiled water and 50% ethanol-soaked

Sample Preparation:

Sampleprep ID:SP001264
Sampleprep Summary:The extracts were centrifuged (15 min, 13,000 rpm) at 4oC and 3 mL of supernatant was collected. The supernatant was frozen at -80oC and then dried using a vacuum centrifugal evaporator (CHRiST, Alpha 2-4/LSC, Germany). The dried residues were separately dissolved in 3 mL of distilled water. A 600 µL extract was filtered (Millipore Amicon® ULTRA 3 kDa) and filtrates were collected. 3-(Trimethylsilyl) propanesulfonic acid (50 µL) pre-dissolved in D2O (an internal standard) was subsequently added to 450 µL of the filtrates. Finally, aliquots (480 µL) of each extract were transferred to a 5 mm NMR tube (Norell, Morganton, NC, USA) for NMR analysis.

Analysis:

MB Sample ID:SA082598
Analysis ID:AN001981
Analysis Type:NMR
Num Factors:4
Num Metabolites:52
Units:ppm

NMR:

NMR ID:NM000150
Analysis ID:AN001981
Instrument Name:Bruker AV III 600 MHz NMR spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
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