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MB Sample ID: SA082699
| Local Sample ID: | M-52 |
| Subject ID: | SU001257 |
| Subject Type: | Animal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001257 |
| Subject Type: | Animal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M-52 | SA082699 | FL012568 | Skeletal Muscle (Gastrocnemius) | Tissue |
| M-52 | SA082699 | FL012568 | Vascular catherization/Pseudomonas aeruginosa infusion | Challenge |
Collection:
| Collection ID: | CO001251 |
| Collection Summary: | Tissue was harvested and flash frozen in a liquid nitrogen cooled |
| Sample Type: | Ileum;Lung;Liver;Jejunum;Skeletal muscle |
Treatment:
| Treatment ID: | TR001272 |
| Treatment Summary: | Fraction of cardiac tissue weighed (~25 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50 % water, 0.3% formic acid) at a standard concentration of 25 mg/475 microlieters (mcl) buffer then fully homogenized on ice for 20 seconds and placed on dry ice/stored at -80C |
Sample Preparation:
| Sampleprep ID: | SP001265 |
| Sampleprep Summary: | The samples were crash deprotonized by methanol precipitation and spiked with D27-deuterated myristic acid (D27-C14:0) as an internal standard for retention-time locking and dried. The trimethylsilyl-D27-C14:0 standard retention time (RT) was set at 16.727 min. Reactive carbonyls were stabilized at 50C with methoxyamine hydrochloride in dry pyridine. Metabolites were made volatile with TMS groups using N-methyl-N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50C. |
Combined analysis:
| Analysis ID | AN001982 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 N GC |
| Column | Agilent DB5-MS (15m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | Peak values (Log transformed) |
Chromatography:
| Chromatography ID: | CH001430 |
| Chromatography Summary: | GC/MS methods follow previous studies using a 6890 N GC connected to a 5975 Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122-5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
| Instrument Name: | Agilent 6890 N GC |
| Column Name: | Agilent DB5-MS (15m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001835 |
| Analysis ID: | AN001982 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | NA |
| Ion Mode: | POSITIVE |
