Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA086224

Local Sample ID:	Sup_Fig4A-1
Subject ID:	SU001286
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Subject:

Subject ID:	SU001286
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
Sup_Fig4A-1	SA086224	FL012854	Vitamin D-	Group

Collection:

Collection ID:	CO001280
Collection Summary:	C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University
Sample Type:	Cecum

Treatment:

Treatment ID:	TR001301
Treatment Summary:	C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University

Treatment ID:

TR001301

Treatment Summary:

C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University

Sample Preparation:

Sampleprep ID:	SP001294
Sampleprep Summary:	Extract metabolites from cecal contents, then run samples. Pleas see the NMR protocol doc attached to this file
Sampleprep Protocol Filename:	NMR_protocol.docx

Analysis:

MB Sample ID:	SA086224
Analysis ID:	AN002032
Analysis Type:	NMR
Num Factors:	4
Num Metabolites:	6
Units:	umol/mg

NMR:

NMR ID:	NM000151
Analysis ID:	AN002032
Instrument Name:	Bruker Avance II
Instrument Type:	FT-NMR
NMR Experiment Type:	1D-1H
Spectrometer Frequency:	600