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MB Sample ID: SA088395
| Local Sample ID: | CA209038-8-48_week 6 |
| Subject ID: | SU001303 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001303 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CA209038-8-48_week 6 | SA088395 | FL012979 | week 6 | Time point |
| CA209038-8-48_week 6 | SA088395 | FL012979 | 1 | OS_Censor (1 means the time is a censoring time and 0 means a failure time in OS) |
Collection:
| Collection ID: | CO001297 |
| Collection Summary: | Serum was collected at the specified time-points by centrifugation at 4000g for 4 minutes at 25°C within 2 hours of collection. Samples were frozen immediately and stored at or below -20°C for up to two months followed by storage at -80°C. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR001318 |
| Treatment Summary: | Serum samples were obtained from 78 participants in the the nivolumab monotherapy arm of CA209-038 (NCT01621490), a multi-institutional, multiarm prospective trial investigating the pharmacodynamics of nivolumab in patients with unresectable or metastatic melanoma. |
| Treatment Protocol ID: | NCT01621490 |
Sample Preparation:
| Sampleprep ID: | SP001311 |
| Sampleprep Summary: | Serum samples (10 µL) were extracted using 90 µL of 74.9:24.9:0.2 (v/v/v) acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (0.2 ng/µL valine-d8, Isotec; 0.2 ng/µL phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 min, 9000g, 4ºC) and the supernatants were transferred to autosampler vials with de-activated inserts (Waters). Factors: OS_Censor (1 means the time is a censoring time and 0 means a failure time in Overall Survival) |
Combined analysis:
| Analysis ID | AN002053 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters Atlantis HILIC (150 x 2.1mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6495 QQQ |
| Ion Mode | POSITIVE |
| Units | Log10(Peak Area) |
Chromatography:
| Chromatography ID: | CH001492 |
| Chromatography Summary: | Extracts (10 µL) were injected onto a 150 x 2.1 mm Atlantis HILIC column (Waters). The column was eluted isocratically at a flow rate of 250 µL/min with 5% mobile phase A (10 mM ammonium formate and 0.1% formic acid in water) for 1 minute followed by a linear gradient to 40% mobile phase B (acetonitrile with 0.1% formic acid) over 10 minutes |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Waters Atlantis HILIC (150 x 2.1mm) |
| Column Temperature: | 30 |
| Flow Rate: | 250 µL/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001905 |
| Analysis ID: | AN002053 |
| Instrument Name: | Agilent 6495 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | MS data were acquired using multiple reaction monitoring. Retention times, mass transitions, and collision energies were determined using authentic reference standards. Other MS parameters were: ion spray voltage, 3.0 kV; source temperature, 200°C; nozzle voltage, 500 V; gas flow, 14 L/min; nebulizer, 40 psi; sheath gas, 250°C; sheath gas flow, 1 L/min; iFunnel high pressure RF, 90; and low pressure RF, 90. Raw data were processed using MassHunter software (Agilent, Santa Clara, CA) for automated peak integration. The peak areas of 106 targeted metabolites were manually reviewed for quality of integration and compared against standard reference standards to confirm identities. |
| Ion Mode: | POSITIVE |
