Return to study ST001253 main page
MB Sample ID: SA091209
| Local Sample ID: | 7021 |
| Subject ID: | SU001321 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | db/db |
| Age Or Age Range: | 10 months |
| Weight Or Weight Range: | 70-80gr |
| Gender: | Male |
| Animal Animal Supplier: | Jackson B6.BKS(D)-Leprdb/J (stock number:000697) homozygous db/db mice |
| Animal Housing: | Indiana University LARC |
| Animal Light Cycle: | 12:12 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001321 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | db/db |
| Age Or Age Range: | 10 months |
| Weight Or Weight Range: | 70-80gr |
| Gender: | Male |
| Animal Animal Supplier: | Jackson B6.BKS(D)-Leprdb/J (stock number:000697) homozygous db/db mice |
| Animal Housing: | Indiana University LARC |
| Animal Light Cycle: | 12:12 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 7021 | SA091209 | FL013129 | db/m | diabetes |
| 7021 | SA091209 | FL013129 | ZT17 | time of day |
Collection:
| Collection ID: | CO001315 |
| Collection Summary: | Upon euthanasia, blood was collected via cardiac puncture in EDTA tubes and stored on ice until plasma isolation. Plasma isolation was done at 4oC for 20 min 800g. Plasma immediately was frozen in liquid ntirogen and stored at -80oC until sended to Metabolon Inc |
| Sample Type: | Blood (plasma) |
| Collection Method: | cardiac puncture |
| Collection Location: | INDIANAPOLIS |
| Collection Duration: | 1hr |
| Volumeoramount Collected: | 500ul |
| Storage Conditions: | -80℃ |
| Collection Vials: | EDTA vials |
| Storage Vials: | 0.5ml microcentrifuge tubes |
| Collection Tube Temp: | ice |
Treatment:
| Treatment ID: | TR001336 |
| Treatment Summary: | Animals were fed ad lib for 10 months with chow diet (2018SX, Harlan, 18% protein, 5% fat, 5% fiber). We obtained plasma samples at ZT5 and ZT17. Mice were held in Controlled-humidity, temperature and light environment facility at Indiana University, Indianapolis |
Sample Preparation:
| Sampleprep ID: | SP001329 |
| Sampleprep Summary: | Frozen tissues were kept in 0.5 ml round-bottomed Eppendorf tubes at -80oC and send to Metabolon Inc |
| Sampleprep Protocol ID: | Metabolon |
| Sampleprep Protocol Filename: | Method |
| Processing Storage Conditions: | On ice |
| Sample Resuspension: | 100ul |
Combined analysis:
| Analysis ID | AN002079 | AN002080 | AN002081 | AN002082 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | VolNormImp Area counts | VolNormImp Area counts | VolNormImp Area counts | VolNormImp Area counts |
Chromatography:
| Chromatography ID: | CH001516 |
| Chromatography Summary: | LC/MS Pos Early: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), accidic, positive ion conditions |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001517 |
| Chromatography Summary: | LC/MS Pos Late: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), acidic positive ion conditions |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001518 |
| Chromatography Summary: | LC/MS negative: Waters Acquity , Reversed phase, separate column BEH C18 (100 X2mm, 1.7um), basic negative ion optimized conditions |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001519 |
| Chromatography Summary: | LC/MS Polar: Instrument Waters Acquity , Reversed phase, Column: BEH Amide (150 X2mm, 1.7um), |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001930 |
| Analysis ID: | AN002079 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Heated electrosplay ionization HESI-II |
| Ion Mode: | POSITIVE |
| MS ID: | MS001931 |
| Analysis ID: | AN002080 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Heated electrosplay ionization HESI-II |
| Ion Mode: | POSITIVE |
| MS ID: | MS001932 |
| Analysis ID: | AN002081 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Heated electrosplay ionization HESI-II |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001933 |
| Analysis ID: | AN002082 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Heated electrosplay ionization HESI-II |
| Ion Mode: | NEGATIVE |
