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MB Sample ID: SA092649
| Local Sample ID: | GCM_P0_3+ |
| Subject ID: | SU001342 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | E18-P90 |
| Weight Or Weight Range: | not measured |
| Height Or Height Range: | not measured |
| Gender: | Pooled |
| Animal Animal Supplier: | The Jackson Laboratory |
| Animal Housing: | standard |
| Animal Light Cycle: | standard 12h |
| Animal Feed: | standard grain-based |
| Animal Water: | standard unlimited |
| Animal Inclusion Criteria: | random |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001342 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | E18-P90 |
| Weight Or Weight Range: | not measured |
| Height Or Height Range: | not measured |
| Gender: | Pooled |
| Animal Animal Supplier: | The Jackson Laboratory |
| Animal Housing: | standard |
| Animal Light Cycle: | standard 12h |
| Animal Feed: | standard grain-based |
| Animal Water: | standard unlimited |
| Animal Inclusion Criteria: | random |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| GCM_P0_3+ | SA092649 | FL013266 | GCM | Fraction |
| GCM_P0_3+ | SA092649 | FL013266 | P0 | Age |
Collection:
| Collection ID: | CO001336 |
| Collection Summary: | Growth cone from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP) were collected. These fractions were generated through established differential centrifugation techniques and lipid extraction from established protocols: 1-Estrada-Bernal, A. et al. Functional complexity of the axonal growth cone: a proteomic analysis. PloS one 7, e31858 (2012). 2-Pfenninger et al 1983 paper (Pfenninger, K. H., Ellis, L., Johnson, M. P., Friedman, L. B. & Somlo, S. Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization. Cell 35, 573-584 (1983) |
| Sample Type: | Brain |
Treatment:
| Treatment ID: | TR001357 |
| Treatment Summary: | No Treatment |
Sample Preparation:
| Sampleprep ID: | SP001350 |
| Sampleprep Summary: | We performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). |
Combined analysis:
| Analysis ID | AN002112 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Accela 600 |
| Column | Thermo Acclaim C30 (150 x 2.1mm,3m) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | main area |
Chromatography:
| Chromatography ID: | CH001544 |
| Instrument Name: | Accela 600 |
| Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3m) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001965 |
| Analysis ID: | AN002112 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were ran in positive and negative ion mode |
| Ion Mode: | UNSPECIFIED |
