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MB Sample ID: SA093021
| Local Sample ID: | D14_7- |
| Subject ID: | SU001345 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 2 Months |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001345 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 2 Months |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| D14_7- | SA093021 | FL013315 | Sonication | Exposure |
| D14_7- | SA093021 | FL013315 | Day Fourteen | Days Post Exposure |
Collection:
| Collection ID: | CO001339 |
| Collection Summary: | Mice were exposed to a controlled sonication pulse and optic nerve samples were collected at 1 day, 7 days, and 14 days post sonication. Lipids were then extracted, resuspended in Chloroform:Methanol (1:1) and processed through untargeted liquid chromatography Q-Exactive Orbitrap tandem mass spectrometry (LC-MS/MS). |
| Sample Type: | optic nerve |
Treatment:
| Treatment ID: | TR001360 |
| Treatment Summary: | Mice were exposed to a 500msec sonication shock at the supraorbital rim that did not create any skull fractures nor cause death. Mice were sacrificed at 1 day, 7 days, or 14 days post exposure for lipid analysis of optic nerves. |
Sample Preparation:
| Sampleprep ID: | SP001353 |
| Sampleprep Summary: | As described in collection |
Combined analysis:
| Analysis ID | AN002113 | AN002114 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 600 | Thermo Accela 600 |
| Column | Thermo Acclaim C18 (150 x 2.1mm,3m) | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Main area | Main area |
Chromatography:
| Chromatography ID: | CH001547 |
| Instrument Name: | Thermo Accela 600 |
| Column Name: | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001968 |
| Analysis ID: | AN002113 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | A Heated Electrospray Ionization Source (HESI) was operated at a spray voltage of 4.4kV, a HESI vaporization temperature of 275°C, a sheath gas pressure of 45 arbitrary units, and an auxillary gas flow of 15 arbitrary units. The ion transfer tube was kept at a temperature of 350°C. The scan range was set at 150-1500 m/z. |
| Ion Mode: | POSITIVE |
| MS ID: | MS001969 |
| Analysis ID: | AN002114 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | A Heated Electrospray Ionization Source (HESI) was operated at a spray voltage of 4.4kV, a HESI vaporization temperature of 275°C, a sheath gas pressure of 45 arbitrary units, and an auxillary gas flow of 15 arbitrary units. The ion transfer tube was kept at a temperature of 350°C. The scan range was set at 150-1500 m/z. |
| Ion Mode: | NEGATIVE |
