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MB Sample ID: SA093489
| Local Sample ID: | Cage3_Wk8 |
| Subject ID: | SU001352 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 5wks-13wks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (Bar Harbor, ME) |
| Animal Light Cycle: | 12hr light 12hr dark |
| Animal Feed: | LabDiet 5001 (PMI International, Inc, St. Louis, MO) ad libitum |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001352 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 5wks-13wks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (Bar Harbor, ME) |
| Animal Light Cycle: | 12hr light 12hr dark |
| Animal Feed: | LabDiet 5001 (PMI International, Inc, St. Louis, MO) ad libitum |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Cage3_Wk8 | SA093489 | FL013379 | placebo | Condition |
| Cage3_Wk8 | SA093489 | FL013379 | 8 | Timepoint (weeks) |
Collection:
| Collection ID: | CO001346 |
| Collection Summary: | Urine: 18 hour collection from metabolic cages. At the end of each collection urine volume was measured and centrifuged (2000xg, 10 mins, °C). Internal standard was added equivalent to 10% of sample volume, aliquots were transferred into screw-top cryotubes and stored at -80°C. WB: cardiac puncture after anesthesia. Collected by cardiac puncture and transferred to a microcentrifuge tube containing sodium heparin in an ice water bath. Stored @ -80°C within 10 minutes of collection |
| Collection Protocol Filename: | MouseUrineCollection,Processing_MetabolicCages.V1.5.pdf |
| Collection Protocol Comments: | Urine Collected 4 times over 8 weeks, each collection over 18 hours |
| Sample Type: | Urine |
| Collection Method: | From Metabolic cages |
| Collection Frequency: | 2 weeks |
| Collection Duration: | 18hr |
| Storage Conditions: | -80℃ |
| Collection Vials: | Sterile 2mL microcentrifuge tubes |
| Storage Vials: | Sterile 2mL microcentrifuge tubes |
| Collection Tube Temp: | Room temp |
Treatment:
| Treatment ID: | TR001367 |
| Treatment Summary: | For treated mice: CFZ dissolved in sesame oil, added to chow, as 0.03% to powdered feed mix, chow provided ad libitum |
| Treatment Compound: | Clofazamine (C8895; Sigma-Aldrich, St. Louis, MO) |
| Treatment Route: | CFZ dissolved in sesame oil, added to chow, chow provided ad libitum |
| Treatment Dose: | 0.03% CFZ to powdered feed mix |
| Treatment Doseduration: | 8 weeks |
| Treatment Vehicle: | sesame oil (Shirakiku, Japan) |
| Animal Acclimation Duration: | 1 week |
| Animal Endp Euthanasia: | exsanguination while deeply anethetied with an intraperitoneal injection of ketamine (100mg/kg) and xlazine (10mg/kg) |
Sample Preparation:
| Sampleprep ID: | SP001360 |
| Sampleprep Summary: | Urine: centrifugation (2000xg, 10mins, 4°C) before NMR |
| Processing Method: | centrifugation |
| Processing Storage Conditions: | 4℃ |
| Sample Spiking: | DSS-IS2 (Chenomx, Inc) |
Analysis:
| MB Sample ID: | SA093489 |
| Analysis ID: | AN002121 |
| Laboratory Name: | NMR Metabolomics Laboratory |
| Analysis Type: | NMR |
| Acquisition Date: | 1/29/2014, 5/15/14, 5/19/14, 5/20/14, 5/21/14, 5/22/14, 5/23/14, 10/23/14, 11/11/14, 11/24/14 |
| Operator Name: | Larisa Yeomans |
| Detector Type: | 1D-1H |
| Num Factors: | 8 |
| Num Metabolites: | 34 |
| Units: | µM |
NMR:
| NMR ID: | NM000153 |
| Analysis ID: | AN002121 |
| Instrument Name: | Varian 500 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| NMR Comments: | Arrayed for water saturation frequency and 90deg. pulse width for each sample |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | ONE-Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming), manual when necessary. |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Offset Frequency: | around -178Hz |
| Chemical Shift Ref Cpd: | DSS-d6 IS-2 (Chenomx) |
| Temperature: | 25 |
| Number Of Scans: | 32 |
