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MB Sample ID: SA093861

Local Sample ID:82629
Subject ID:SU001364
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Subject:

Subject ID:SU001364
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
82629SA093861FL013432RMLInocula
82629SA093861FL01343260 dpiTimepoint

Collection:

Collection ID:CO001359
Collection Summary:Brains were harvested and fash-frozen with dry ice
Sample Type:Brain

Treatment:

Treatment ID:TR001379
Treatment Summary:Wild type FVB mice were subjected to intracerebral injection to be inoculated with 30ul of 1% (w.v) RML brain homogenate or normal brain homogenate. At 30, 60, 90, 120 and 150 dpi, a sub-set of RML and NBH inoculated animals were euthanized.

Sample Preparation:

Sampleprep ID:SP001372
Sampleprep Summary:Half brains were homogenized with Dounce tissue homogenizers to make 10% (w/v) homogenate. The homogenate is then subject to ultracentrifuge at 100,000xg for 1h at 4°C, the resulting supernatant was neutralized with Tris buffer (0.5M, pH6.8) one tenth the volume of the supernatant. Then the neutralized supernatant is passed through 3kDa molecular weight cut-off filters at 4000 rpm for 45 mins at 4°C. Filtered supernatant was tried at SpeedVac Vacuum Concentrator at low heating until try pellets were received. Try pellets were then dissolved with 530ul of water and 60 ul of D2O containing 5mM of DSS. A final volume of 600ul for each sample was achieved.

Analysis:

MB Sample ID:SA093861
Analysis ID:AN002146
Analysis Type:NMR
Num Factors:10
Num Metabolites:50
Units:uM

NMR:

NMR ID:NM000156
Analysis ID:AN002146
Instrument Name:INOVA NMR spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:500MHz
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