Return to study ST001295 main page

MB Sample ID: SA094121

Local Sample ID:233_69
Subject ID:SU001369
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:32-67
Gender:Male and female
Human Race:Caucasian (C) and African American (AA)
Human Ethnicity:non-hispanic
Human Inclusion Criteria:Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc)
Human Exclusion Criteria:Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent.

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU001369
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:32-67
Gender:Male and female
Human Race:Caucasian (C) and African American (AA)
Human Ethnicity:non-hispanic
Human Inclusion Criteria:Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc)
Human Exclusion Criteria:Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent.

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
233_69SA094121FL013455ControlCondition

Collection:

Collection ID:CO001364
Collection Summary:WB samples (12 mL each) were collected by direct venipuncture or from an indwelling line into K2 EDTA-containing tubes. Samples were flash frozen and stored at -80C.
Sample Type:Blood (whole)
Collection Method:direct venipuncture or indwelling line
Collection Frequency:once
Volumeoramount Collected:12mL
Storage Conditions:-80℃
Collection Vials:K2 EDTA-containing Vacutainer tubes (BD 134367863; Becton-Dickinson, Franklin Lakes, NJ USA)
Additives:K2 EDTA

Treatment:

Treatment ID:TR001384
Treatment Summary:No treatment. Sepsis and non-sepsis controls had platelets isolated. Mitochondrial function was measured via mitochondrial oxygen consumption (mOCR)

Sample Preparation:

Sampleprep ID:SP001377
Sampleprep Summary:Methanol:cholorform precipitation
Sampleprep Protocol ID:WB methanol Chlorform precipitation
Sampleprep Protocol Filename:233.5.1_-_HAPS_WB_MeOHCHCl3_Precipitation
Processing Method:precipitation
Processing Storage Conditions:On ice
Extraction Method:Methanol chloroform precipitation
Extract Enrichment:Lyophilization
Extract Storage:-80℃
Sample Resuspension:500uL 50mM sodium phosphate buffer in D2O
Sample Spiking:4.99 mM DSS internal standard

Analysis:

MB Sample ID:SA094121
Analysis ID:AN002156
Laboratory Name:University of Michigan BioNMR core
Analysis Type:NMR
Acquisition Date:04/05/2018, 04/09/2018, 04/10/2018
Software Version:TopSpin 4.0.7
Operator Name:Larisa Yeomans, Jae Hyun Kim
Num Factors:2
Num Metabolites:31
Units:µM

NMR:

NMR ID:NM000158
Analysis ID:AN002156
Instrument Name:Bruker 18.8 Tesla (800 MHz) NMR spectrometer ascend
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
NMR Comments:Arrayed for water saturation frequency and 90deg. pulse width for each sample
Standard Concentration:10% (~0.5mM)
Spectrometer Frequency:800 MHz
NMR Probe:Triple resonance inverse detection TCI cryoprobe
NMR Solvent:D20
NMR Tube Size:5mm
Shimming Method:Auto shim (gradient shimming)
Pulse Sequence:noesygppr1d
Water Suppression:saturation at 80 Hz induced field strength
Pulse Width:5.5ms
Offset Frequency:around -178Hz
Presaturation Power Level:80db
Chemical Shift Ref Cpd:DSS-d6
Temperature:25
Number Of Scans:32
  logo