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MB Sample ID: SA094782
| Local Sample ID: | HC_3 |
| Subject ID: | SU001390 |
| Subject Type: | Plant |
| Subject Species: | Nannochloropsis gaditana |
| Taxonomy ID: | 72520 |
| Genotype Strain: | NIES 2587 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001390 |
| Subject Type: | Plant |
| Subject Species: | Nannochloropsis gaditana |
| Taxonomy ID: | 72520 |
| Genotype Strain: | NIES 2587 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HC_3 | SA094782 | FL013532 | Treatment | Condition |
Collection:
| Collection ID: | CO001385 |
| Collection Summary: | Marine microalgae Microchloropsis gaditana NIES 2587 is procured from Microbial Culture Collection, National Institute for Environmental Studies (NIES), Tsukuba, Japan. The strain was grown in minimal medium F/2 (Guillard and Ryther, 1962) under a light regime of 16:8 h and an illumination of 150 µmol m−2 s−1 photosynthetically active radiation (PAR) in a multi-cultivator MC 1000-OD (Photon Systems Instruments, Czech Republic) with a flow rate of 800 mL min-1 with continuous bubbling of air at 24 °C. |
| Sample Type: | Algae |
Treatment:
| Treatment ID: | TR001405 |
| Treatment Summary: | Microchloropsis gaditana cells were grown in a Multicultivator in the presence of very-low CO2 (300 ppm) and high CO2 (30,000 ppm) for 9 days with an illumination intensity of 150 uE. |
| Treatment Compound: | CO2 |
Sample Preparation:
| Sampleprep ID: | SP001398 |
| Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN002191 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 7890A |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001606 |
| Chromatography Summary: | GC-triple quadrupole analysis was performed on an HP-5 gas chromatograph with standard liners containing glass wool in split mode (1:5) at 250°C injector temperature. The GC was operated at constant flow of 1 ml/min helium on a 30-m, 0.25-mm i.d., 0.25-μm HP-5 column, a start temperature of 60°C, 3 min isothermal, temperature ramping by 5°C/min to 180°C, 3 min isothermal and finally temperature ramping of 10°C/min to 310°C. |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002038 |
| Analysis ID: | AN002191 |
| Instrument Name: | Agilent 7890A |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | NA |
| Ion Mode: | POSITIVE |
