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MB Sample ID: SA095549
| Local Sample ID: | 171212_03 |
| Subject ID: | SU001395 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001395 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 171212_03 | SA095549 | FL013557 | 24 h | Time |
Collection:
| Collection ID: | CO001390 |
| Collection Summary: | Biomass samples were withdrawn for quenching with 60% methanol. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001410 |
| Treatment Summary: | The GC/MS data were firstly converted to mzXML format and imported into R. The data were then deconvolved by using eRah package. A total number of 612 features were detected and after removing features with over 20% RSD in the QCs. |
Sample Preparation:
| Sampleprep ID: | SP001403 |
| Sampleprep Summary: | Metabolome were extracted from biomass snap-freezing in liquid nitrogen with 80% methanol and centrifuge at 15,000 x g -9, three times. The supernatant was dried with speed vacuum for 12 h and subsequently the samples were derivatized with methoxyamination followed by trimethylsylylation. |
Combined analysis:
| Analysis ID | AN002197 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | Agilent 7200 |
| Column | Agilent VF-5ms (30 m x 0.25 mm, 0.25 µm) |
| MS Type | ESI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 7890A |
| Ion Mode | UNSPECIFIED |
| Units | IU |
Chromatography:
| Chromatography ID: | CH001611 |
| Instrument Name: | Agilent 7200 |
| Column Name: | Agilent VF-5ms (30 m x 0.25 mm, 0.25 µm) |
MS:
| MS ID: | MS002043 |
| Analysis ID: | AN002197 |
| Instrument Name: | Agilent 7890A |
| Instrument Type: | GC-TOF |
| MS Type: | ESI |
| MS Comments: | The mass spectrum was collected for the range of 50-550 m/z with an acquisition rate of 5 spectra/s and an acquisition time of 200 ms/spectrum |
| Ion Mode: | UNSPECIFIED |
